Since its introduction almost 20 years ago, phage display technology has revolutionized approaches to the analysis of biomedical problems, quickly impacting the fields of immunology, cell biology, biotechnology, pharmacology, and drug discovery. In Antibody Phage Display: Methods and Protocols, Second Edition,expert researchers explore the latest in this cutting-edge technology, providing an invaluable resource that will guide readers in the design and executionof experiments based around antibody phage display. Chapters present a wide range of methods of isolating recombinant antibodies from phage display libraries, examine how the targets recognized by antibodies of interest can be identified, discuss the identification and exploitation of antibodies that can entercells and bind to cytosolic targets, and include novel approaches to the expression of recombinant antibodies. Fully updated from the first edition to offer cutting-edge methods for antibody phage display Wide-ranging methods presented by an international authorship Innovative applications of antibody phage display applicable to all areas of bioscience Comprehensive protocols to guide novices and experienced investigators alike INDICE: From the contents Antibody phage display – overview of a powerful technology that has quickly translated to the clinic.- Design and constructionof synthetic phage-displayed Fab libraries.- Affinity isolation of antigen-specific circulating B cells for generation of phage display-derived human monoclonal antibodies.- Construction of phage antibody repertoires from the blood of West Nile virus infected donors.- Anti-ß2GP-I and anti-prothrombin antibodies generated by phage display.- The generation and selection of single-domain, V region libraries from nurse sharks.- The isolation of scFvs against small target molecules.- Display and selection of scFv antibodies on HEK-293T cells.- Isolation of scFvs that inhibit the NS3 protease of Hepatitis C virus by a combination of phage display and a bacterial genetic screen.- Guided selection methods through chain shuffling.- Sequential antigen panning for selection of broadly cross-reactive HIV-1 neutralizing human monoclonal antibodies.
- ISBN: 978-1-60327-301-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 225
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés