Elucidating cellular physiology at the level of the individual cell is at the forefront of the biological sciences. New techniques are making it possible to move from static to dynamic studies of intracellular enzyme behavior as well as increasing our ability to assay an expanding repertoire of enzymatic activities driving cell behavior. This compendium provides an overview of the cutting edge technologies being used to delve into the intracellular signaling responsible for cellular phenotype and function. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesUpdated release includes the latest information on Enzyme Activity in Single Cells INDICE: 1. Spatial and temporal resolution of caspase waves in single Xenopus eggs during apotosis James E. Ferrell and Jr. 2. Spatial and temporal organization of metabolic complexes in cells Songon An 3. Measuring cellular efflux and biomolecular delivery: Synthetic approaches to imaging and engineering cells Markita Landry 4. Slide-based, single-cell enzyme assays Birgitta Knudsen 5. Single-cell assays using integrated continuous-flow microfluidics Chia-Hung Chen 6. High-throughput screening of single-cell lysates Florian Hollfelder 7. Microfluidic capture of single cells for drug resistance assays Paul C.H Li 8. Microfluidic tools for measuring intra- and interclonal heterogeneity in protease activity Eiusik Yoon 9. Nanocapillary-based electrochemical detection for single cells Dechen Jiang 10. Chemical cytometry by automated capillary electrophoresis Nancy Allbritton 11. Microelectrophoretic single-cell measurements with microfluidic devices Chris Culbertson 12. Chemical probes for spatially-resolved MS enzyme measurements of single cells Raymond Moellering 13. Extracellular matrix nanoarrays for SAMDI-MS single-cell enzyme assays Milan Mrksich 14. Tracking embryonic development with single-cell CE-MS Peter Nemes 15. Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells Julia Laskin 16. Investigation of Protein Function in Single Cells Aldo Jesorka
- ISBN: 978-0-12-817090-8
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/11/2019
- Nº Volúmenes: 1
- Idioma: Inglés