Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesUpdated release includes the latest information on phosphatases INDICE: Computation 1. Empirical Valence Bond Simulations of the Evolution of Enzyme Function Shina Caroline Lynn Kamerlin and Miha Purg 2. QM/MM free energy and kinetic isotope analysis of phosphoryl transfer in enzymes Qiang Cui Structure, mechanism and evolution of phosphatases 3. How to define rapid motions in pumping pyrophosphatases Adrian Goldman 4. The evolution of K+-independence in pyrophosphatases Adrian Goldman 5. Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases Karen N. Allen 6. Investigation of Nucleotide Loading and Effector Binding of K-Ras Vadim Gaponenko Substrate specificity in phosphatases 7. Phosphatases from the Amidohydrolase Superfamily Frank M. Raushel 8. Comparison of Enzyme States by Principal Component Analysis of NMR Spectra using TREND NMR Software Steven R. Van Doren 9. Assessment and impacts of phosphorylation on protein flexibility of the phosphohexomutases Lesa Beamer 10. Molecular tools for studying the impact of cis/trans prolyl isomerization on RNA polymerase II CTD phosphatase activity and specificity Yan Jessie Zhang Signaling and Regulation 11. Quantitative analysis of intracellular phosphatase activity of histidine kinases Ann M. Stock 12. Measuring the Activities of Two-Component Regulatory System Phosphatases Robert B. Bourret 13. Biochemical Characterization of PGAM5, an Atypical Protein Phosphatase Peter Tipton 14. Measuring phosphatidic acid phosphatase activity of purified lipins Thurl Harris 15. Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities Milan Mrksich 16. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation Phillip A. Cole
- ISBN: 978-0-12-813881-6
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/09/2018
- Nº Volúmenes: 1
- Idioma: Inglés