The Immunological Synapse Part A, Volume 173 in the Methods in Cell Biology series provides state-of-the-art methods for the study of the immunological synapse. This first volume covers various aspects on T cell and natural killer (NK) cell synapses, including imaging polarized granule release using TIRF microscopy, analysis of actin reorganization and centrosome polarization, redirected degranulation, live cell-imaging to quantify cytotoxic and chemotactic dynamics, quantification of interactions between APCs and T cells, assessment of membrane lipid state at the immunological synapse, proteomic analysis and imaging of NK-tumor cell interaction, evaluation of natural killer cell effector functions against breast cancer cells derived from human tumor tissue, evaluation of chimeric antigen receptor (CAR)-modified cell immunological synapse quality using the glass-supported planar lipid bilayer, and monitoring potency of therapeutic CAR T cells. It also includes one chapter on quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers, and one chapter on the murine antibody-dependent cellular phagocytosis assay. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of Immunological Synapse INDICE: 1. Imaging polarized granule release at the cytotoxic T cell immunological synapse using TIRF microscopy: control by polarity regulators Marie Juzans, Céline Cuche, Vincenzo Di Bartolo, and Andrés Alcover 2. Analysis of centrosomal area actin reorganization and centrosome polarization upon lymphocyte activation at the immunological synapse Sara Fernández-Hermira, Irene Sanz-Fernández, Marta Botas, Víctor Calvo, and Manuel Izquierdo 3. P815-based redirected degranulation assay to study human NK cell effector functions Iñigo Terrén, Gabirel Astarloa-Pando, Ainhoa Amarilla-Irusta, and Francisco Borrego 4. Cytotoxic and Chemotactic Dynamics of Natural Killer Cells Quantified by Live-cell Imaging Yanting Zhu and Jue Shi 5. Quantification of interaction frequency between antigen-presenting cells and T cells by conjugation assay Ondrej Cerny 6. Assessment of membrane lipid state at the natural killer cell immunological synapse Jordan Orange and Yu Li 7. Study of the Effects of NK-Tumor Cell Interaction by Proteomic Analysis and Imaging Chiara Lavarello, Paola Orecchia, Andrea Petretto, Massimo Vitale, Claudia Cantoni, and Monica Parodi 8. Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers Eliana Stanganello, Magdalena Brkic, Steven Zenner, Ines Beulshausen, Ute Schmitt, and Fulvia Vascotto 9. Quantification of lymphocytic choriomeningitis virus specific T cells and LCMV viral titers Melanie Grusdat, Catherine Dostert, and Dirk Brenner 10. An in vitro model to monitor natural killer cell effector functions against primary breast cancer Nicky A. Beelen, Femke A. I. Ehlers, Loes F. S. Kooreman, Gerard M. J. Bos, and Lotte Wieten 11. Standardized Protocol for the Evaluation of Chimeric Antigen Receptor (CAR)-modified Cell Immunological Synapse Quality using the Glass-supported Planar Lipid Bilayer Jong Hyun Cho, Wei-chung Tsao, Alireza Naghizadeh, and Dongfang Liu 12. Potency Monitoring of Therapeutic Car T Cells Dongrui Wang, Xin Yang, Agata Xella, Lawrence A. Stern, and Christine Brown
- ISBN: 978-0-323-90154-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 362
- Fecha Publicación: 20/01/2023
- Nº Volúmenes: 1
- Idioma: Inglés