Methods in Enzymology serial highlights new advances in the field with this new volume presenting interesting chapters. Each chapter is written by an international board of authors. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in Methods in Enzymology serials Updated release includes the latest information on Modifications and Targeting of Protein Termini INDICE: 1. Optimizing purification and activity assays of N-terminal methyltransferase complexesChristine Schaner Tooley, Haley Parker and John Tooley2. In vitro reconstitution of hierarchical steps of Arg/N-degron pathwayHyun Kyu Song and Bong Heon Kim3. Identification of N-degrons and N-recognins by using peptide-pull-downs combined with quantitative proteomicsFranziska Müller and Tanja Bange4. A decoupled Virotrap approach to study the interactomes of N-terminal proteoformsKris Gevaert, Annelies Bogaert and Tessa Van de Steene5. Monitoring ADO-dependent proteolysis in cells using fluorescent reporter proteinsThomas P. Keeley6. Site-specific a-N-terminal methylation on peptides through chemical and enzymatic methodsRong Huang and Ying Meng7. Monitoring the interactions between N-degrons and N-recognins of the Arg/N-degron pathwayYong Tae Kwon and Ah Jung Heo8. Characterization and chemical modulation of p62/SQSTM1 as an autophagic N-recognin of the Arg/N-degron pathwayYong Tae Kwon, Chang Hoon Ji, Min Ju Lee and Su Bin Kim9. Characterization of C-degron-mediated protein degradationHsueh-Chi S. Yen, Chi-Wei Yeh, Wei-Chieh Huang, Shu-Yu Hsu, Ching-Yu Chu, Lo-Tung Lee and Kai-Han Lin10. Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiaeAndreas Bachmair, Nikola Winter, Aida Kozlic, Theresia Telser, Lilian Nehlin, Charlene Dambire and Michael J. Holdsworth11. Deformylation of nascent chains on the ribosomeMarina V. Rodnina, Lena Bögeholz, Evan Mercier and Wolfgang Wintermeyer12. High-throughput dissection of protein degradation signals with fluorescent timersAnton Khmelinskii and Ka-Yiu Edwin Kong13. Affinity isolation and biochemical characterisation of N-degron ligands using the N-recognin, ClpSKaye N. Truscott and David A. Dougan14. Probing the effects of N-terminal acetylation on a-synuclein structure, aggregation and toxicityJanet R. Kumita, Rosie Bell and Michele Vendruscolo15. Peptide CoA conjugates for in situ proteomics profiling of acetyltransferase activitiesIris Finkemeier, Jürgen Eirich, Julia Sindlinger, Stefan Schön and Dirk Sch
- ISBN: 978-0-443-15772-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/08/2023
- Nº Volúmenes: 1
- Idioma: Inglés