The AGT Cytogenetics Laboratory Manual

INDICE: Contributing Authors .Preface .Acknowledgements .1. The Cell and Cell Division Margaret J. Barch and Helen J. Lawce .1.1 The Cell .1.2 The Cell Cycle .1.3 Recombinant DNA Techniques .1.4 The Human Genome .References .2. Cytogenetics: An Overview Helen J. Lawce and Michael G. Brown .2.1 Introduction .2.2 History of Human Cytogenetics .2.3 Cytogenetics Methods .2.4 Slide–Making .2.5 Chromosome Staining .2.6 Chromosome Microscopy/Analysis .References .Contributed Protocols .3. Peripheral Blood Cytogenetic Methods Helen J. Lawce and Michael G. Brown .3.1 Using Peripheral Blood for Cytogenetic Analysis .3.2 Special Uses of Peripheral Blood Culture .3.3 Peripheral Blood Constituents .3.4 Special Handling .3.5 Cell Culture Equipment and Supplies .3.6 Harvesting Peripheral Blood Cultures .3.7 Chromosome Analysis of Peripheral Blood .3.8 Storage of Fixed Specimens .Acknowledgements .References .Contributed Protocols .4. General Cell Culture Principles and Fibroblast Culture Debra F. Saxe, Kristin M. May and Jean H. Priest .4.1 Definitions of Cell Culture .4.2 Basic Considerations in Cell Culture .4.3 Fibroblast Culture .Glossary .References .Additional Readings .Contributed Protocols .5. Prenatal Chromosome Diagnosis Kristin M. May, Debra F. Saxe and Jean H. Priest .5.1 Introduction .5.2 Amniotic Fluid .5.3 Culture of Amniotic Fluid .5.4 Analysis of Amniotic Fluid .5.5 Chorionic Villus Sampling (CVS) .5.6 Analysis of Chorionic Villus .References .Contributed Protocols .6. Chromosome Stains Helen J. Lawce .6.1 Introduction .6.2 Chromosome Banding Methods .6.3 5 –bromodeoxyuridine (BrdU) Methodologies .6.4 T–Banding/CT–Banding .6.5 Antibody Banding and Restriction Endonuclease Banding .6.6 Destaining Slides .6.7 FISH DAPI Bands .6.8 Sequential Staining .6.9 Acknowledgements .References .Contributed Protocols .7. Human Chromosomes: Identification and Variations Helen J. Lawce and Luke Boyd .7.1 Understanding the Basics .7.2 Description of Human Chromosome Shapes .7.3 Determination of G–Banded Chromosome Resolution .Glossary .References .8. ISCN: The Universal Language of Cytogenetics Marilyn Schaeffer Arsham and Lisa G. Shaffer .8.1 The Language .8.2 Karyotype .8.3 Numerical Events .8.4 Structural Events .8.5 Derivative Chromosomes (der) .8.5.4 Multiple Events Derivative .8.6 Symbols of Uncertainty .8.7 Random Versus Reportable .8.8 Multiple Cell Lines .8.9 Fluorescence In Situ Hybridization (FISH) .8.10 Microarray (arr) and Region Specific Assay (rsa) .Conclusion .References .9. Constitutional Chromosome Abnormalities Kathleen Kaiser–Rogers .9.1 Numerical Abnormalities .9.2 Structural Rearrangements .References .10. Genomic Imprinting R. Ellen Magenis .10.1 Introduction .10.2 Human Genomic Disease and Imprinting .10.3 Germ Cell Tumors – UPD and Imprinting .References .Glossary of Terms .11. Cytogenetic Analysis of Hematologic Malignant Disease Nyla Heerema .11.1 Introduction .11.2 Leukemia .11.3 B– and T–Cell Lymphoblastic Neoplasms .11.4 Lymphomas .11.5 Laboratory Practices .Acknowledgements .References .Glossary .Contributed Protocols .12. Cytogenetic Methods and Findings in Human Solid Tumors Marilu Nelson .12.1 Introduction .12.2 Processing Tumor Specimens .12.3 Recurrent Cytogenetic Abnormalities .12.4 Molecular Genetics and Cytogenetics Techniques .Conclusion .References .Contributed Protocols .13. Chromosome Instability Syndromes Yassmine Akkari .13.1 Introduction .13.2 Fanconi Anemia .13.3 Bloom Syndrome .13.4 Ataxia Telangiectasia .13.5 Nijmegen Breakage Syndrome .13.6 Immunodeficiency, Centromeric Instability, and Facial Anomalies (ICF) Syndrome .13.7 Roberts Syndrome] .13.8 Werner Syndrome .13.9 Rothmund–Thomson Syndrome] .13.10 Proficiency Testing] .Glossary] .References .Contributed Protocols .14. Microscopy and Imaging Margaret J. Barch and Helen J. Lawce .14.1 The Standard Microscope .14.2 Brightfield Microscopy .14.3 Fluorescence Microscopy .14.4 Specialized Microscopy .14.5 Capturing the Microscopic Image .References .15. Computer Imaging Christine E. Haessig .15.1 Introduction .15.2 Techniques to Improve Karyogram Image Quality .15.3 Metaphase Preparation .15.4 Microscopy .15.5 Image Capture .15.6 Enhancement .15.7 Advanced Contrast .15.8 Macro Programming .15.9 FISH Imaging .15.10 Printing .15.11 Quality Control .15.12 Archiving .References .16. Fluorescence in situ Hybridization (FISH) Helen J. Lawce and Jeff Sanford .16.1 Introduction .16.2 Clinical Applications of FISH Probes .16.3 Deletion/Duplication Probes for Constitutional Abnormalities .16.4 Hematology/Oncology and Solid Tumor Probes .16.5 Sources and Characteristics of Probes Available to the Clinical Cytogenetics Laboratory .16.6 Special Uses of Probes .16.7 Important FISH Probe Adjuvants .16.8 Principles of FISH .16.9 FISH Methods An Overview .16.10 FISH Analysis and Reporting .16.11 FISH Probe Testing and Validation .16.12 FISH for Special Investigation .16.13 Preimplantation Genetic FISH .16.14 Other Applications .16.15 Variants in FISH Signal Patterns .Conclusion .Glossary .References .Contributed Protocols .17. Multicolor FISH (SKY and M–FISH) and CGH Turid Knutsen .17.1 Introduction .17.2 Multicolor FISH (SKY/M–FISH) .17.3 Comparative Genomic Hybridization (CGH) .Conclusion .Acknowledgements .References .Contributed Protocols .18. Genomic Microarray Technologies for the Cytogenetics Laboratory Bhavana J. Dave and Warren G. Sanger .18.1 Introduction .18.2 Applications .18.3 aCGH in a Cytogenetics Laboratory .Conclusion .Acknowledgements .Authors Note .Editors Note .References .19. Mathematics for the Cytogenetics Technologist Patricia K. Dowling .19.1 General Concepts .19.2 Solutions .19.3 Statistical Tools .19.4 Using a Hemacytometer .19.5 Quantification and Purity Determination of DNA Using Spectroscopy .References .Additional Readings .20. Selected Topics on Safety, Equipment Maintenance, and Compliance for the Cytogenetics Laboratory Helen Jenks and Janet Krueger .20.1 Introduction .20.2. Biological Hazard Safety .20.3 Chemical Safety .20.4 Fire Safety .20.5 Electrical Safety .20.6 Disaster Plan .20.7 Equipment Operation, Maintenance and Safety .20.8 Ergonomics .20.9 Regulatory Considerations .Acknowledgements .References .Contributed Protocols .21. A System Approach to Quality Peggy J. Stupca and Sheryl A. Tran .21.1 Quality System .21.2. Process Management .21.3 Documents and Records .21.4 Assessments .21.5 Continual Improvement .References .Contributed Protocols .22. Laboratory Management Mervat Ayad and Adam Sbeiti .22.1 Introduction .22.2 Management Concepts and Functions .22.3 Personnel Management .22.4 Quality Management and Control .22.5 Budget Development and Monitoring .Conclusion .References .Suggested Reading .23. Laboratory Information System Peining Li and Richard Van Rheeden .23.1 Historical Perspective .23.2 General Description of LIS .23.3 LIS in Cytogenetics Laboratories .23.4 Trends for the Future LIS .Acknowledgements .References .24. Animal Cytogenetics Marlys L. Houck, Teri L. Lear and Suellen J. Charter .24.1 Introduction .24.2 Domestic Animal Fertility .24.3 Captive Management .24.4 Wildlife Conservation .24.5 General Sample Collection Considerations .24.6 Fibroblast Cell Culture .24.7 Peripheral Blood Culture .24.8 Chromosome Analysis .24.9 Molecular and Comparative Cytogenetics .Acknowledgements .Glossary .References .Contributed Protocols .25. Online Genetic Resources & References Wahab A. Khan .25.1 Resource Information

• ISBN: 978-1-119-06122-9
• Editorial: Wiley–Blackwell
• Encuadernacion: Cartoné
• Páginas: 1192
• Fecha Publicación: 05/01/2017
• Nº Volúmenes: 1
• Idioma: Inglés