Regulated Cell Death Part A: Apoptotic Mechanisms

Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization scienceRegulated Cell Death Part A & Part B contains sections on such topics as apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches INDICE: 1. Examining the Molecular Mechanism of Bcl-2 Family Proteins at Membranes by Fluorescence Spectroscopy.2. Photoreactive Stapled Peptides to Identify and Characterize BCL-2 Family Interaction Sites by Mass Spectrometry3. The Structural Biology of BH3-only proteins4. How to Analyze Mitochondrial Morphology in Healthy Cells and Apoptotic Cells in Caenorhabditis elegans5. Apoptosis Initiation Through the Cell-Extrinsic Pathway6. Using RNAi Screening Technologies to Interrogate the Extrinsic Apoptosis Pathway7. Caspase Enzymology and Activation Mechanisms8. Turning ON Caspases with Genetics and Small Molecules9. A Multi-Pronged Approach for Compiling a Global Map of Allosteric Regulation in the Apoptotic Caspases10. Measuring Caspase Activity in vivo11. Single-Molecule Sensing of Caspase Activation in Live Cells via Plasmon Coupling Nanotechnology12. In vivo Monitoring of Caspase Activation Using a Fluorescence Resonance Energy Transfer-Based Tfluorescent Probe13. Global Analysis of Cellular Proteolysis by Selective Enzymatic Labeling of Protein N-termini14. Complementary Methods for the Identification of Substrates of Proteolysis15. Phospholipid Scrambling on the Plasma Membrane16. Studying Apoptosis in the Zebrafish

• ISBN: 978-0-12-417158-9
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 514
• Fecha Publicación: 07/07/2014
• Nº Volúmenes: 1
• Idioma: Inglés