The innate immune response is a crucial component of early resistance to infection, and it is now revealing increasing levels of complexity. The ability tomodify the genome in vivo, has facilitated understanding of complex interactions between leucocytes and other components of the immune system, and phenotype-driven strategies using chemical mutagenesis have placed another powerful weapon in the armamentarium. In, Leucocytes: Methods and Protocols provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes and their products. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Leucocytes: Methods and Protocols seeks to aid scientists in further study into the immune system and leucocytes. Provides detailed protocols and practical advice on a variety of modern approaches to the study of leucocytes. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from the experts. INDICE: ENU Based Phenotype Driven Screening. Detection and Quantificationof Cytokines and Other Biomarkers. FLOW CYTOMETRY ANALYSIS OF CELL CYCLING AND PROLIFERATION IN MOUSE HEMATOPOIETIC STEM AND PROGENITOR CELLS. Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo. Analyzing Cell Death Events in Cultured Leukocytes. Towards a four-dimensional view of neutrophils. Isolation of Human and Mouse Neutrophils Ex vivo and In vitro.MEASUREMENT OF OXIDATIVE BURST IN NEUTROPHILS. Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates. The Macrophage. Generation and Characterization of MacGreen Mice, the Cfs1r -EGFP Transgenic Mice. Generation of Mouse Bone Marrow-derived Macrophages. Isolation and Differentiation of Monocytes-macrophages from Human Blood. In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages. Measuring the Inflammasome. Arginine and Macrophage Activation. Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation. Detection of Human and Mouse Granzyme B Activity in Cell Extracts. T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation. Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry.
- ISBN: 978-1-61779-526-8
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 320
- Fecha Publicación: 28/02/2012
- Nº Volúmenes: 1
- Idioma: Inglés