Stem cells in regenerative medicine: methods and protocols

As regenerative medicine involves replacing diseased cells, tissues or organs, or repairing tissues in vivo, the manipulation of stem cells underlie its goals. In Stem Cells in Regenerative Medicine: Methods and Protocols, leading experts in the field provide an updated representation of the landscape of stem cell-based therapies in a wide spectrum of tissue systems and ontogenic stages, from the isolation and culture of stem cells to their actual use in vivo. Written in the highly successful Methods in Molecular Biology™ series format, these chapters include brief introductions to the topic, lists of the necessary materials and reagents, readily reproducible, step-by-step laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Provides both in vivo and in vitro assays Includes protocols for both adult and embryonic stem cells INDICE: From the contents Derivation and Manipulation of Murine Embryonic Stem Cells.- Patterning Mouse and Human Embryonic Stem Cells using Micro-Contact Printing.- Efficient Gene Knockdowns in Human Embryonic Stem Cells using Lentiviral Based RNAi.- Measurement of Cell-Penetrating Peptide-Mediated Transduction of Adult Hematopoietic Stem Cells.- Stem Cell Sources for Regenerative Medicine.- Investigating the Interactions between Hemopoietic Stem Cells and their Niche: Methods for the Analysis of Stem Cell Homing and Distribution within the Marrow following Transplantation.- Ex Vivo Megakaryocyte Expansion and Platelet Production from Human Cord Blood Stem Cells.- Ex vivo Generation of Human Red Blood Cells: A New Advance in Stem Cell Engineering.- Isolation and Manipulation of Mammalian Neural Stem Cells in Vitro.- Isolation, Expansion, andDifferentiation of Mouse Skin-Derived Precursors.

• ISBN: 978-1-58829-797-6
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 355
• Fecha Publicación: 01/01/2009
• Nº Volúmenes: 1
• Idioma: Inglés