CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesUpdated release includes the latest information on the CRISPR-Cas Enzymes INDICE: 1. CRISPR bioinformatics 2. A method for one-step assembly of Class 2 CRISPR arrays 3. Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems 4. Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system 5. Site-specific fluorescent labeling of individual proteins within CRISPR complexes 6. Fluorescence-based methods for measuring target interference by CRISPR-Cas systems 7. Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry 8. ChIP-based assays to measure Cas protein binding to DNA in vivo 9. Live-cell super-resolution microscopy of Cascade-mediated DNA surveillance 10. In vitro assembly of Crispr-Cas Type III-A system 11. Cyclic oligoadenylate signalling by type III CRISPR systems 12. A pipeline for characterization of Cas9 orthologues 13. Preparation and use of Cas12a RNPs for genome editing experiments 14. Discovery and Mechanistic Studies of A Thermophilic Cas9 towards Gene Editing and Cancer Diagnostics Applications 15. Kinetic characterization of Cas9 enzymes 16. Single-molecule FRET studies of Cas9 endonuclease 17. CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA 18. dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) 19. CRISPR-Cas10 assisted editing of virulent bacteriophages 20. Reconstituted in vitro CRISPR adaptation and its detection by PCR 21. Quantitative deep sequencing of CRISPR-Cas spacer acquisition
- ISBN: 978-0-12-816760-1
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 01/02/2019
- Nº Volúmenes: 1
- Idioma: Inglés