This first book to focus on all principles and aspects of AFM in liquid phaseis perfectly structured, making it easy-to-follow for non-AFM specialists. Atthe same time, it is an excellent introduction for researchers wishing to usethis important technique for evaluating biological material and biological applications.From the contents:ˆ— AFM: Basic conceptˆ— Dynamic modes in liquidsˆ— Force spectroscopyˆ— Forces in liquidsˆ— Single molecule force spectroscopyˆ— High resolution imaging of biological materialˆ— Imaging of force-distance curvesˆ— High speed AFM for observing dynamic processesˆ— Theory and fundamentalsˆ— Combination of AFM with optical methodsˆ— Biological applications ˆ— Electrochemical AFMˆ— Manipulation and lithographyAn optimum balance for chemists,physicists, materials scientists, and biologists, as well as analytical and medicinal chemists. INDICE: Preface XIIIList of Contributors XVPart I General Atomic Force Microscopy 11 AFM: Basic Concepts 3Fernando Moreno-Herrero and Julio Gomez-Herrero1.1 Atomic Force Microscope: Principles 31.2 Piezoelectric Scanners 51.3 Tipsand Cantilevers 81.4 Force Detection Methods for Imaging in Liquids 151.5 AFMOperation Modes: Contact, Jumping/Pulsed, Dynamic 191.6 The Feedback Loop 241.7 Image Representation 251.8 Artifacts and Resolution Limits 28Acknowledgments 32References 322 Carbon Nanotube Tips in Atomic Force Microscopy with Applications to Imaging in Liquid 35Edward D. de Asis, Jr., Joseph Leung, and Cattien V. Nguyen2.1 Introduction 352.2 Fabrication of CNT AFM Probes 372.3 ChemicalFunctionalization 442.4 Mechanical Properties of CNTs in Relation to AFM Applications 462.5 Dynamics of CNT Tips in Liquid 502.6 Performance and Resolutionof CNT Tips in Liquid 58References 613 Force Spectroscopy 65Arturo M. Baró3.1 Introduction 653.2 Measurement of Force Curves 673.3 Measuring Surface Forces by the Surface Force Apparatus 703.4 Forces between Macroscopic Bodies 713.5Theory of DLVO Forces between Two Surfaces 713.6 Van der Waals Forces - the Hamaker Constant 723.7 Electrostatic Force between Surfaces in a Liquid 723.8 Spatially Resolved Force Spectroscopy 763.9 Force Spectroscopy Imaging of Single DNA Molecules 783.10 Solvation Forces 793.11 Hydrophobic Forces 813.12 Steric Forces 813.13 Conclusive Remarks 83Acknowledgments 83References 834 Dynamic-Mode AFM in Liquid 87Takeshi Fukuma and Michael J. Higgins4.1 Introduction 874.2 Operation Principles 884.3 Instrumentation 904.4 Quantitative Force Measurements 974.5 High-Resolution Imaging 1104.6 Summary and Future Prospects 116References 1175 Fundamentals of AFM Cantilever Dynamics in Liquid Environments 121Daniel Kiracofe, John Melcher, and Arvind Raman5.1 Introduction 1215.2 Reviewof Fundamentals of Cantilever Oscillation 1225.3 Hydrodynamics of Cantileversin Liquids 1235.4 Methods of Dynamic Excitation 1265.5 Dynamics of Cantilevers Interacting with Samples in Liquids 1405.6 Outlook 150References 1506 Single-Molecule Force Spectroscopy 157Albert Galera-Prat, Rodolfo Hermans, Rub n HervÃís, Ãüngel Gómez-Sicilia, and Mariano Carrión-VÃízquez6.1 Introduction 1576.2 AFM-SMFS Principles 1596.3 Dynamics of Adhesion Bonds 1656.4 Specific versus Other Interactions 1696.5 Steered Molecular Dynamics Simulations 1766.6 Biological Findings Using AFM-SMFS 1776.7 Concluding Remarks 182Acknowledgments 182Disclaimer 182References 1827 High-Speed AFM for Observing Dynamic Processesin Liquid 189Toshio Ando, Takayuki Uchihashi, Noriyuki Kodera, Mikihiro Shibata, Daisuke Yamamoto, and Hayato Yamashita7.1 Introduction 1897.2 Theoretical Derivation of Imaging Rate and Feedback Bandwidth 1907.3 Techniques Realizing High-Speed Bio-AFM 1927.4 Substrate Surfaces 2007.5 Imaging of Dynamic Molecular Processes 2037.6 Future Prospects of High-Speed AFM 2067.7 Conclusion 207References 2078 Integration of AFM with Optical Microscopy Techniques 211Zhe Sun, Andreea Trache, Kenith Meissner, and Gerald A. Meininger8.1 Introduction 2118.2 Combining AFM with IRM and TIRF microscopy 2178.3 Combining AFM and FRET 2218.4 FRET-AFM 2228.5 Sample Preparation and Experiment Setup 223References 225Part II Biological Applications 2319 AFM Imaging in Liquid of DNA and Protein-DNA Complexes 233Yuri L. Lyubchenko9.1 Overview: the Study of DNA at Nanoscale Resolution 2339.2 Sample Preparation for AFM Imaging of DNA and Protein-DNA Complexes 2349.3 AFM of DNA in Aqueous Solutions 2369.4 AFM Imaging of Alternative DNA Conformations 2399.5 Dynamics of Protein-DNA Interactions 2479.6 DNA Condensation 2539.7 Conclusions 254Acknowledgments 254References 25510 Stability of Lipid Bilayers as Model Membranes: Atomic Force Microscopy and Spectroscopy Approach 259Lorena Redondo-Morata, Marina In´es Giannotti, and Fausto Sanz10.1 Biological Membranes 25910.2 Mechanical Characterization of Lipid Membranes 26310.3 Future Perspectives 279References 27911 Single-Molecule Atomic Force Microscopy of Cellular Sensors 285Jürgen J. Heinisch and Yves F. Dufrêne11.1 Introduction 28511.2 Methods 28811.3 Probing Single Yeast Sensors in Live Cells 29211.4 Conclusions 302Acknowledgments 303References 30312 AFM-Based Single-Cell Force Spectroscopy 307Clemens M. Franz and Anna Taubenberger12.1 Introduction 30712.2 Cantilever Choice 31012.3 Cantilever Functionalization 31012.4Cantilever Calibration 31112.5 Cell Attachment to the AFM Cantilever 31112.6 Recording a Force-Distance Curve 31312.7 Processing F-D Curves 31512.8 Quantifying Overall Cell Adhesion by SCFS 31712.9 SFCS with Single-Molecule Resolution 32012.10 Dynamic Force Spectroscopy 32112.11 Measuring Cell-Cell Adhesion 32512.12 Conclusions and Outlook 326References 32713 Nanosurgical Manipulation of Living Cells with the AFM 331Atsushi Ikai, Rehana Afrin, Takahiro Watanabe-Nakayama,and Shin-ichi Machida13.1 Introduction: Mechanical Manipulation of Living Cells 33113.2 Basic Mechanical Properties of Proteins and Cells 33113.3 Hole Formation on the Cell Membrane 33213.4 Extraction of mRNA from Living Cells33413.5 DNA Delivery and Gene Expression 33513.6 Mechanical Manipulation of Intracellular Stress Fibers 33813.7 Cellular Adaptation to Local Stresses 34313.8 Application of Carbon Nanotube Needles 34413.9 Use of Fabricated AFM Probeswith a Hooking Function 34613.10 Membrane Protein Extraction 34813.11 Future Prospects 350Acknowledgments 350References 350Index 355
- ISBN: 978-3-527-32758-4
- Editorial: Wiley-VCH
- Encuadernacion: Cartoné
- Páginas: 402
- Fecha Publicación: 04/04/2012
- Nº Volúmenes: 1
- Idioma: Inglés