Recent work has revealed that stabilizing G-quadruplexes in telomeric DNA inhibits telomerase activity, providing impetus for the development of G-quartet-interacting drugs, while G-quartet-containing oligonucleotides have been recognized as a potent class of aptamers effective against STAT3 and other transcription factors implicated in oncogenesis, proving these guanine-quartets to be a vital and rich area for future study. In 'G-Quadruplex DNA: Methods and Protocols', experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols,and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 'G-Quadruplex DNA: Methods and Protocols 'promises to be a usefulresource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest inthe exciting implications of G-quadruplex DNA. Includes coverage of a wide range of methodologies to study G-quadruplex structures Provides detailed protocols which ensure successful re-recreation of experimental procedures Supplies step-by-step instructions in order to enable researchers to set up their own high throughput screens for G-quadruplex ligands Explores computational approaches for the detection of G-quadruplex-forming structures Examines state of theart physical, chemical, and biological methods Contains tricks of the trade and valuable troubleshooting advice INDICE: G-Quadruplexes: From Guanine Gels to Chemotherapeutics.- MolecularModeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes.- Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential.- Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis.- Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay.- Real-time Observation of G-Quadruplex Dynamics Using Single Molecule FRET Microscopy.- Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures.- 2-Aminopurine as a Probe for Quadruplex Loop Structures.- Assessing DNA Structures with 125I Radioprobing.- Monitoring the Temperature Unfolding of G-Quadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques.- Probing Telomeric G-Quadruplex DNA Structures in Cells with in vitro Generated Single-Chain Antibody Fragments.- Detection of G-Quadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC.- Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography.- Quantifying Interactions between G-Quadruplex DNA and Transition Metal Complexes.- G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands.
- ISBN: 978-1-58829-950-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 275
- Fecha Publicación: 01/12/2009
- Nº Volúmenes: 1
- Idioma: Inglés