Microarray technologies have arisen to a robust and widely accepted tool in inflammation research. Already the first microarray publications in 1996 as well as later ground-breaking publications were dedicated to topics in inflammation research. Since then, many opportunities but also hurdles specifically linked to the application of microarrays to inflammation topics have been envisaged and partly solved. This book summarizes the most advanced technical aspects covering all steps for a thorough application of microarrays to inflammation topics – from sample generation to data analysis. In addition selected examplesof successful applications of microarrays in major fields of inflammation research are presented. After reading the book a researcher or clinician should be in the position to plan, perform and analyse or to critically review microarray experiments related to inflammation research. Summarizes the most advancedtechnical aspects from sample generation to data analysis INDICE: From the contents 1 Microarray and inflammation: an introduction.-2 Methods and protocols for the generation of gene expression profiles in inflammation research.- 2.1 Sample preparation: Whole Blood.- Sample preparation:Comparison of PBMCs and blood cell subsets.- Sample preparation: Skin.- Sample preparation: Adipose tissue.- Sample preparation: Samples isolated by Fluorescent Activated Cell Sorting (FACS).- Sample preparation: Samples isolated by Magnetic Activated Cell Sorting (MACS).- Sample preparation: Logistics, storage and shipping of samples.- 2.2 Single and rare cell analysis – amplification methods.- T7 based amplification protocols.- Global PCR based protocols.- 2.3 Microarray platforms and comparability thereof.- 2.4 Data generation and interpretation: Algorithms, Software, data bases.
- ISBN: 978-3-7643-8333-6
- Editorial: Birkhaüser
- Encuadernacion: Cartoné
- Páginas: 260
- Fecha Publicación: 01/05/2008
- Nº Volúmenes: 1
- Idioma: Inglés