With new technologies emerging and old technologies being reimagined, the field of hematopoietic stem cell (HSC) research continues to grow rapidly in pursuit of improved therapies for many blood diseases. Updating and building upon the thorough first edition's foundation, Hematopoietic Stem Cell Protocols, Second Edition provides up-to-date protocols developed in the HSC field. A team of leading researchers supply this volume with in-depth, readily reproducible methods for effective characterization of HSC and their developmental potential. An introductory chapter also hints at potential techniques that may developin the future. Following the format of the successful Methods in Molecular Biology™ series, each protocol offers step-by-step laboratory instructions, lists of the necessary equipment, and tips on troubleshooting and avoiding known pitfalls. Provides detailed flow cytometry protocols for thorough analysis of enriched HSC populations. Offers a variety of transplantation approaches to measure HSC function in vivo. Details retroviral and lentiviral transduction procedures and analysis of integration sites. Describes assays using embryonic andadult tissues to derive HSC INDICE: From the contents The Expanding Tool Kit for Hematopoietic Stem Cell Research.- Isolation of Quiescent Murine Hematopoietic Stem cells by HomingProperties.- Mobilization of Hematopoietic Stem and Progenitor cells (HSPC) in Mice.- Transmigration of Human CD34+ cells.- Flow Cytometry Based Cell CycleMeasurement of Mouse Hematopoietic Stem and Progenitor Cells.- Analysis of Apoptosis in HSC by Flow Cytometry.- In vitro Hematopoietic Differentiation of Murine Embryonic Stem (ES) Cells.- Hematopoietic Development of Human EmbryonicStem Cells in Culture.- In vitro Human T Cell Development Directed by Notch-ligand Interactions.- In vitro Assays for Cobblestone Area Forming Cells, LTC-IC, and CFU-C.- HSC Transplant in Mice by Intra Femoral Injection.- Hematopoietic Stem Cell Transplant into Non-myeloablated W/Wv mice to Detect Steady-state Engraftment Defects.
- ISBN: 978-1-58829-868-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 360
- Fecha Publicación: 01/02/2008
- Nº Volúmenes: 1
- Idioma: Inglés