Mass spectrometry analysis for protein-protein interactions and dynamics

This reference presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Methods include deuterium exchange, covalent labeling, footprinting, plistex, and computational approaches. The coverage of footprinting and plistex sets the book apart from the competition. INDICE: Chapter 1: Overview of Mass Spectrometry Technologies for Examining Protein Structure: Current and Future Directions. Chapter 2: Hydrogen Exchange Mass Spectrometry: Principles and Capabilities. Chapter 3: Covalent labeling Methods for Examining Protein Structure and Protein Interactions. Chapter 4:Complementary Methods for Structure Determination: Hydroxyl Radical-Mediated Footprinting and Deuterium Exchange Mass Spectrometry as Applied to Serpin Structure . Chapter 5: Deuterium Exchange Approaches for Examining Protein Interactions: Case Studies of Complex Formation. Chapter 6: Hydrogen/Deuterium Exchange Studies of Viruses. Chapter 7: Use of Enhanced Peptide Amide Hydrogen/Deuterium Exchange-Mass Spectrometry (DXMS) in the Examination of Protein-Protein Interactions. Chapter 8: Cross-Linking as a tool to Examine Protein Complexes:Examples of Cross-Linking Strategies and Computational Modeling. Chapter 9: Complex Formation in the Actin Cytoskeleton: Cross-linking Tools to Define Actin Protein Structure and Interactions. Chapter 10: Computational Approaches to Examining Protein-Protein Interactions: Combining Experimental and Computational Data in the Era of Structural Genomics. Chapter 11: Studies of Intact Proteins and Protein Complexes: ESI MS Approaches. Chapter 12: Two Approaches to Mass Spectrometric Protein Foot Printing: PLIMSTEX and FPOP.

• ISBN: 978-0-470-25886-6
• Editorial: John Wiley & Sons
• Encuadernacion: Cartoné
• Páginas: 296
• Fecha Publicación: 29/08/2008
• Nº Volúmenes: 1
• Idioma: Inglés