Significant advancements have been made in the study of chromatin structure and function over the past fifty years but none as spectacular as those made inthe last decade due to the development of novel techniques and the ability tosequence large stretches of DNA. In Chromatin Protocols, Second Edition, expert researchers delineate these cutting-edge techniques via step-by-step laboratory methods and protocols, which encompass a wide array of topics from the isolation of nucleosomes, assembly of nucleosomes and study of the basic chromatin structure to detailed analysis of histone modifications and chromatin function. Written in the highly successful Methods in Molecular Biology™ series style, chapters include brief introductions to the subjects, lists of the necessary materials and reagents, readily reproducible protocols, and Notes sections which highlight tips on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for analysis of chromatin structure and function incorporating the latest techniques Includes detailed, step by step guidelines for the analysis of chromatin structure and its role in transcription, replication and other biological processes INDICE: From the contents Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos.- Analysis of Reconstituted Chromatin Using aSolid-Phase Approach.- In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins.- DNA Methyltransferase Probing of Chromatin Structure within Populations and on Single Molecules.- Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues.- Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin.- Analysis of DNA Topology in Yeast Chromatin.- Preparation and Analysis of Uniquely Positioned Mononucleosomes.- Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy.- Methods to Study Transcription-CoupledRepair in Chromatin.
- ISBN: 978-1-58829-873-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 400
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés