Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems - Part A

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems - Part A, Volume 638, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Sample chapters from this new release include In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe, Using FDAA probes to study cell division in Bacillus subtilis, Chemoenzymatic synthesis of UDP-sugars, Chemical tools for selective activity profiling of bacterial penicillin-binding proteins, Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on retinoid signaling pathways INDICE: 1. In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe Emily P. Balskus 2. Using FDAA probes to study cell division in Bacillus subtilis Michael VanNieuwenhze 3. Chemoenzymatic synthesis of UDP-sugars Catherine L. Grimes 4. Chemical tools for selective activity profiling of bacterial penicillin-binding proteins Erin Carlson 5. Chemical Probes Reveal and Extraseptal Mode of Cross-linking in Staphylococcus Aureus David Speigel 6. Protocol for synthesis and purification of ultrapure salicyl-AMS, to remove a toxic synthetic impurity that we have discovered Derek Tan 7. A Probe-Enabled Approach for Function-Dependent Isolation and Characterization of Microbiome Subpopulations Aaron Wright 8. Biochemical analysis of NlpC/p60 peptidoglycan hydrolase activity Howard Hang 9. Nanocapsules for chemical control of membrane-initiated steroid signaling Yamuna Krishnan 10. Single-cell imaging of nascent transcription in whole-mount embryos Matt Good 11. Reversible Re-programing of Cell-Cell Interactions Carsten Wagner 12. Chemically Induced Dimerizers Based on Human Antibody James A. Wells 13. phosphine-triggered decaging of proteins via the Staudinger reduction Alex Deiters 14. Optical control of sphingosine-1-phosphate formation and function Dirk Trauner 15. Expanding the Substrate Selectivity in SNAP/CLIP-Tagging of Intracellular Targets Lei Zhu 16. Genetic code expansion to incorporate photocaged selenocysteine in mammalian cells Eranthie Weerapana 17. Light controlled protein sequestration in living cells using gibberellic acid derivative Richard Wombacher 18. Protein dimerizer tools based on caged trimethoprim David Chenoweth 19. Site-specific antibody fragment conjugates for targeted imaging Ross Wang 20. Phosphate sensing probes Valerie Christine Pierre 21. A Single Tool to Monitor Multiple Protein-Protein Interactions of the Escherichia coli Acyl Carrier Protein Michael D. Burkart

• ISBN: 978-0-12-820145-9
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/06/2020
• Nº Volúmenes: 1
• Idioma: Inglés