Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems - Part B

Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems - Part B, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology seriesIncludes the latest information on retinoid signaling pathways INDICE: 1. Fluorogenic detection of protein aggregates in live cells using the AggTag method Xin Zhang 2. Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection Cliff Stains 3. Chemical Tools for Multicolor Protein FRET with Tryptophan James Petersson 4. Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition Yitzhak Tor 5. Temporal profiling establishes a dynamic S-palmitoylation cycle Brent Martin 6. Solvation-guided design of fluorescent probes for discrimination of amyloids Jerry Yang 7. Oncogenic and Circadian Effects of Small Molecules Directly and Indirectly Targeting the Core Circadian Clock Michelle E. Farkas 8. Chemical Tools for Selective Activity Profiling of Endogenously Expressed MMP-14 in Multicellular Models Matthew Bogyo 9. Genetically encoding quinoline reverses chromophore charge and enables fluorescent protein brightening in acidic vesicle Lei Wang 10. Florescent amino acids for biological spectroscopy and microscopy Feng Gai 11. Dissecting contributions of catalytic and reader domains in regulation of histone demethylation Danica Fujimori 12. Traceless and Chemoselective Amine Bioconjugation via Phthalimidine Formation in Native Protein Modification Xuechen Li 13. Chemical synthesis of di-ubiquitin modified histones for further biochemical studies Changlin Tian 14. Chemical Tools to Investigate Mechanisms Associated with HSP90 and HSP70 in Disease Gabriela Chiosis 15. Fluorescent Isoindole Crosslink (FlICk) Chemistry: A Rapid, User-friendly Stapling Reaction David M. Perrin 16. Selective Protein N-terminal Labeling with N-Hydroxysuccinimide Esters Daniel Dempsey 17. Covalent live-cell labeling of proteins using a photoreactive fluorogen Jennifer Heemstra 18. Photoconvertable dixanthiledene dyes David Chenoweth

• ISBN: 978-0-12-821151-9
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 15/06/2020
• Nº Volúmenes: 1
• Idioma: Inglés