Multidimensional liquid chromatography: theory and applications in industrial chemistry and the life sciences

This book describes the theory, methodology, and applications of multidimensional liquid phase separations using liquid chromatography and, in some cases, capillary electrophoresis. It addresses the applications of MDLC with an emphasis on recent advances that have increased the practicality of using the technique in today's laboratory. The most up to date information in polymer analysis, natural product profiling, metabolic analysis, and proteomics broadens the book's appeal to a wide audience of analytical, pharmaceutical, and life science researchers. INDICE: Preface. 1. Introduction, (Mark R. Schure and Steven A. Cohen). Theory. 2. Elements of the Theory of Multidimensional Liquid Chromatography, (Mark R. Schure). 3. Peak capacity in Two-dimensional Liquid Chromatography (Joe M. Davis). 4. Decoding Complex 2-D Separations (Francseso Dondi, Maria Chiara Pietrogrande, Nicola Marchetti and Attila Felinger). Columns, Instrumentation,and Methods. 5. Instrumentation for Comprehensive Multidimensional Liquid Chromatography (Robert. E. Murphy and Mark R. Schure). 6. Methods Development forComprehensive Multidimensional Liquid Chromatography (Robert E. Murphy and Mark R. Schure). 7. Monolithic Columns and their 2D-HPLC applications (Tohru Ikegami, Hiroshi Aoki, Hiroshi Kimura, Ken Hosoya and Nobuo Tanaka). 8. Ultra-High Pressure Multidimensional Liquid Chromatography (Charles R. Evans and James W. Jorgenson). Life Sciences Applications. 9. Peptidomics (Egidijus Machtejevas, Klaus K. Unger). 10. A Two-dimensional Liquid Mass Mapping Technique for Biomarker (David Lubman, Nathan S. Buchanan, Fred R. Miller, Kathleen Cho, Rong Wu, Steven Goodison, Yanfei Wang, Paweena Kreunin and Timothy J. Barder). 11. Coupled Multidimensional Chromatography and Tandem Mass Spectrometry Systems for Complex Peptide Mixture Analysis (Mike Washburn). 12. Development of Orthogonal 2D-LC Methods for Separations of Peptides (Martin Gilar, Petra Olivova, Amy E. Daly and John C. Gebler). 13. Protein IEX-RP-TOF MS (Steven A. Cohen andScott Berger). 14. Analysis of Enantiomeric Compounds Using Multidimensional Liquid Chromatography (Renee J. Soukup and Dan Armstrong). Multidimensional separations using CE. 15. Two-dimensional capillary electrophoresis for the comprehensive analysis of complex protein mixtures (James R. Kraly, Melissa M. Harwood, Megan Jones and Norman J. Dovici). 16. Two-dimensional HPLC-CE methods for protein/Peptide separation (Haleem Issaq). Industrial Applications. 17. Multidimensional Liquid Chromatography in Industrial Applications (Frank Rittig and Harald Pasch). 18. The Analysis of Surfactants by Multidimensional Liquid Chromatography (Robert E. Murphy and Mark R. Schure.

• ISBN: 978-0-471-73847-3
• Editorial: John Wiley & Sons
• Encuadernacion: Cartoné
• Páginas: 440
• Fecha Publicación: 04/04/2008
• Nº Volúmenes: 1
• Idioma: Inglés