Analytical Ultracentrifugation, the latest volume in Methods in Enzymology, focuses on analytical ultracentrifugation. The scope of this technique has greatly expanded in recent years due to advances in instrumentation, algorithms and software. This volume describes the latest innovations in the field and in the applications of analytical ultracentrifugation in the analysis of macromolecules, macromolecular assemblies, and biopharmaceuticals. Timely contribution that describes a rapidly changing fieldLeading researchers in the fieldBroad coverage: instrumentation, basic theory, data analysis, and applications INDICE: Instrumentation 1. Next generation AUC (2): Multi-wavelength data analysis adds a spectral dimension to hydrodynamic information Borries Demeler 2. Next Generation AUC (1): Multi-Wavelength Detectors for the Analytical Ultracentrifuge add a Spectral Dimension to AUC instrumentation Helmut Coelfen, Kristian Schilling and Borries Demeler Basic Theory and Data Analysis 3. Sedimentation Velocity: Fundamental Principles Walter Stafford and John J. Correia 4. Sedimentation equilibrium Arthur Rowe 5. Auxiliary calculations and publication-quality illustrations for analytical ultracentrifugation data using GUSSI Chad Brautigam 6. Analysis of linked equilibria Aaron Lucius 7. Simulations of AUC Data to Understand the Challenge of Hetero-association John J. Correia 8. Hydrodynamic Modeling Olwyn Byron and Mattia Rocco 9. Problem solving with analytical ultracentrifugation: a systematic approach James Cole Applications 10. Analytical ultracentrifugation analysis of ClpB self-association in dilute and crowded solutions Germán Rivas 11. O6-alkylguanine DNA alkyltransferase Michael Gregory Fried 12. Sedimentation velocity analysis of supramolecular chromatin oligomers using interference optics Jeffrey Hansen 13. Hydrodynamics Models of G-quadruplex Structures Jonathan B. Chaires 14. Carbohydrates Stephen Harding 15. AUC, SANS and SAXS for membrane proteins Christine Ebel 16. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC John Gabrielson and Kelly Arthur 17. Analytical ultracentrifugation and its role in Formulation Development and Research of Biotherapeutical Proteins Steven Shire 18. Sedimentation analysis of the size distribution of amyloid oligomers and fibrils Michael D.W. Griffin and Geoffrey J. Howlett 19. Analytical ultracentrifugation of intrinsically disordered proteins: conformers, conformational changes and ensembles David J. Scott 20. Elucidating complicated assembling systems in biology using size-and-shape analysis of sedimentation velocity data Andrew B. Herr 21. Application of AUC to the Structure & Function of an Essential Oligomeric Enzyme Matthew A. Perugini 22. Role of the analytical ultracentrifuge in dissecting steroid receptor function David L. Bain and Rolando De Angelis 23. Methods for the Analysis of Non-specific Protein-Nucleic Acid Interactions Studied by AUC. Nasib Karl Maluf 24. Interactions in serum John Hill
- ISBN: 978-0-12-802908-4
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 01/10/2015
- Nº Volúmenes: 1
- Idioma: Inglés