Due to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform tobe harnessed for wider drug discovery and point-of-care applications. In Peptide Microarrays: Methods and Protocols, experts in the field provide a cutting-edge view of peptide array technology, its applications, and technical issues. After examining how peptides can characterize proteins and clarify, at the amino acid level, the molecular recognition events in which they are involved, the volume goes on to cover topics such as the production and use of peptide arrays for enzyme and binding motifs characterization, epitope mapping, and diagnostics, the newest technological advancements, as well as software and web tools for the design of peptide arrays and for the analysis of output data. Provides an easily accessible reference volume for peptide microarrays experiments written by leading researchers in the field Details the process of turning biological observations into a high-throughput screening methodology Covers a wide range of peptide microarrays formats and protocols Presents a modular approach to experimental design, screening modalities, detection technologies, andapproaches to data analysis INDICE: From the contents Exploring and Profiling Protein Function with Peptide Arrays.- Peptide Arrays for Enzyme Profiling.- Using Peptide Array to Identify Binding Motifs and Interaction Networks for Modular Domains.- MolecularSimulations of Peptides: A Useful Tool for the Development of New Drugs and for the Study of Molecular Recognition.- Synthesis of Peptide Arrays Using SPOT-Technology and the CelluSpots-Method.- Rapid Identification of Linear ProteinDomain Binding Motifs Using Peptide SPOT Arrays.- Characterization of Kinase Target Phosphorylation Consensus Motifs Using Peptide SPOT Arrays.- CelluSpotsTM: A Reproducible Means of Making Peptide Arrays for the Determination of SH2Domains Binding Specificity.- High Density Peptide Microarrays for Reliable Identification of Phosphorylation Sites and Upstream Kinases.- Epitope Mapping of Human Chromogranin A by Peptide Microarrays.
- ISBN: 978-1-60327-393-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 385
- Fecha Publicación: 01/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés