Great disparities exist between organisms with regard to the relative ease ofchromosomal mutagenesis and manipulation.á In Chromosomal Mutagenesis, aáteamof experts provide a variety of chromosomalámanipulation techniques, including insertional gene disruptions, gene knockouts, stimulated homologous recombination techniques and other novel tools, for both prokaryotic and eukaryotic organisms, and attempt to expand the genetic toolbox beyond model organisms.á Following the format of the highly successful Methods in Molecular Biology™ format, each chapter offers step-by-step laboratory instructions, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Chomosomal Mutagenesis covers state-of-the-art techniques that are staged to expand, if not revolutionize, genetic analysis in the long neglected and relevant cell types.
- ISBN: 978-1-61737-833-1
- Editorial: Springer
- Encuadernacion: Rústica
- Páginas: 236
- Fecha Publicación: 01/01/2008
- Nº Volúmenes: 1
- Idioma: Inglés