This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailedprotocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable. INDICE: Animal Cell Culture - Essential Methods John Davis (Editor) 1. TheCell Culture LaboratorySusan Clarke and Janette Dillon 2. SterilizationPeter Roberts 3. The Microscopy of Living CellsColin Gray and Daniel Zicha 4. Basic Techniques and Media, the Maintenance of Cell Lines, and SafetyJohn Davis5. Serum-Free Culture MediaDavid Jayme and Stephen Gorfien 6. Cryopreservation and Cell BankingGlyn N. Stacey, J. Ross Hawkins and Roland A. Fleck 7. Primary Culture of Specific Cell Types, and the Establishment of Cell LinesKee Woei Ng, Jan-Thorsten Schantz, and Chris Morris 8. CloningJohn Clarke, Alison Porter andJohn Davis 9. The Quality Control of Cell Lines and the Prevention, Detectionand Cure of ContaminationPeter Thraves and Cathy Rowe 10. Systems for CultureScale-UpJennifer Halsall and John Davis 11. Good Laboratory Practice (GLP) inthe Cell Culture LaboratoryBarbara Orton
- ISBN: 978-0-470-66658-6
- Editorial: John Wiley & Sons
- Encuadernacion: Rústica
- Páginas: 376
- Fecha Publicación: 11/03/2011
- Nº Volúmenes: 1
- Idioma: Inglés