Bacterial infections affect world health today as a leading cause of morbidity and mortality. Pathogenic bacteria routinely command a broad spectrum of niches in the human host, making an understanding of pathogenesis mechanisms crucial to the development of prophylactics and treatment for bacterial diseases. A variety of in vitro methods, in vivo animal model systems and cutting-edge genomics assays have arisen in the effort to study bacterial pathogenesis and identify potential therapeutic targets. In Bacterial Pathogenesis, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed tocontrol infections. Collects time-tested, reproducible laboratory protocols, including tricks and hints, written by experts in the field INDICE: From the contents Isolation and Characterization of Lipopolysaccharides.- Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes.- Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus.-Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution.- Detection and Inhibition of Bacterial Cell-Cell Communication.- A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsii.- Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferi.- The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin (PIA) – A Molecular and Biochemical Approach.- Analysis of Staphylococcus aureus Gene Expression during PMN Phagocytosis.- Examining the Vector-Host-Pathogen Interface with Quantitative Molecular Tools.- Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages.
- ISBN: 978-1-58829-740-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 300
- Fecha Publicación: 01/03/2008
- Nº Volúmenes: 1
- Idioma: Inglés