Autophagy and phagocytosis are distinct yet partially morphologically similarprocesses. In Autophagosome and Phagosome, authoritative scientists present easy-to-follow methods on autophagy, a rapidly growing field with a need for standards of assessment, and phagocytosis, a relatively mature field which couldbenefit greatly from updated methods, in order to prompt further explorationsof their similarities and differences. The methods on autophagy allow the reader to find appropriate techniques to identify, monitor, and quantify autophagic processes, while the methods devoted to phagocytosis provide researchers with several modern techniques for in vitro and in vivo studies of phagosomal organelles. Comprehensive and forward-thinking, Autophagosome and Phagosome offers a valuable guide to both cellular processes while inciting researchers to explore the potentially important connections between the two. Autophagosome and phagosome methods for novices and advanced researchers alike Vital for the studies of cancer, aging, neurodegeneration, immunology, and infectious diseases Contains molecular, genetic, biochemical, imaging, cellular, animal, and ultrastructural analyses Autophagy essentials for researchers working on apoptosis and cell death who want to add autophagy to their studies INDICE: From the contents Fine Structure of the Autophagosome.- Methods for Assessing Autophagy and Autophagic Cell Death.- LC3 and Autophagy.- Amino Acid Regulation of Autophagosome Formation.- Autophagic Proteolysis of Long-lived Proteins in Non-liver Cells.- Autophagosomes in Transgenic Mice.- Experimental Control and Characterization of Autophagy in Drosophila.- Analysis of Autophagosome Membrane Cycling by Fluorescence Microscopy.- Protein Trafficking into Autophagosomes.- Sphingolipids in Macroautophagy.- Clearance of Mutant Aggregate-prone Proteins by Autophagy.- Localization and MHC Class II Presentation of Antigens Targeted for Macroautophagy.- Chaperone-mediated Autophagy.- Microautophagy in the Yeast Saccharomyces cerevisiae.- EM Analysis of Phagosomes.- Analysis of Phosphoinositide Dynamics During Phagocytosis Using Genetically Encoded Fluorescent Biosensors.
- ISBN: 978-1-58829-853-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 480
- Fecha Publicación: 01/06/2008
- Nº Volúmenes: 1
- Idioma: Inglés