Recent critical advances in the field of immunotoxicology have led to incredible contributions to basic science, including crucial improvements in human risk assessment. In Immunotoxicity Testing: Methods and Protocols, expert researchers explore these changes, providing the reader with current, lab-ready procedures, along with the corresponding background information that is necessary to identify effective testing approaches for chemicals and drugs. Among the first volumes to meld consideration of immunotoxicity testing strategies with a comprehensive presentation of detailed laboratory protocols, chapters include a description of the evolution of immunotoxicity testing, a look at the importance of immunotoxicity testing for health risk reduction, and ideas concerningthe future of the field. Composed in the highly successful Methods in Molecular Biology(tm) series format, most chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips from the experts on troubleshooting and avoiding known pitfalls. Comprehensive and innovative, Immunotoxicity Testing: Methods and Protocols is a critical, one-stop reference resource for the most important and commonly used laboratory protocols in immunotoxicology. Features step-wise protocols for assays used in immunotoxicity safety evaluation Provides helpful hints to overcome assay problems or increased assay variability Includes chapters covering both clinical evaluation and alternative approaches Offers discussions of new assays likely to impact future testing protocols Presents discussions of age and gender considerations in immune safety testing Incorporates testing approachesdesigned to address emerging immune health risk concerns INDICE: Immunotoxicology Testing: Past and Future.- Developmental Immunotoxicity (DIT): The Why, When and How of DIT Testing.- An In Vivo Approach to Test Immunosensitization by Low Moelcular Weight Compounds.- Risk of Autoimmune Disease: Challenges for Immunotoxicity Testing.- Markers of Inflammation.- Evaluating Macrophages in Immunotoxicity Testing.- Host Resistance Assays Including Bacterial Challenge.- Viral Host Resistance Studies.-Parasite Challenge as Host Resistance Models for Immunotoxicity Testing.- Tumor Challenges in Immunotoxicity Testing.- The T-dependent Antibody Response to Keyhole Limpet Hemocyanin in Rodents.- The T-Dependent Antibody Response to Sheep Erythrocytes.- TheDelayed Hypersensitivity (DTH) Assay Using Protein and Xenogeneic Cell Antigens.- The Cytotoxic T Lymphocyte (CTL) Assay For Evaluating Cell-Mediated Immune Function.- NK Cell Assays in Immunotoxicity Testing.- The Local Lymph Node Assay and Skin Sensitization Test.- Use of Contact Hypersensitivity in Immunotoxicity Testing.- Evaluation of Apoptosis in Immunotoxicity Testing.- DendriticCells in Immunotoxicity Testing.- Evaluating Cytokines in Immunotoxicity Testing.- Flow Cytometry in Preclinical Drug Development.- Expanded Histopathologyof Lymphoid Organs.- Immunotoxicity Testing in Nonhuman Primates.- Fundamentals of Clinical Immunotoxicology.- In Vivo Functional Tests for Assessing Immunotoxicity in Birds.- In Vitro Testing for Direct Immunotoxicity: State-of-the-Art.
- ISBN: 978-1-60761-400-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 428
- Fecha Publicación: 12/08/2009
- Nº Volúmenes: 1
- Idioma: Inglés