DNA microarray technology has revolutionized research in the past decade. Initially an application for mRNA expression studies, the technology now has spread to other applications such as comparative genomic hybridization, SNP and mutation analysis. In DNA Microarrays for Biomedical Research: Methods and Protocols, experts explore these now commonly used applications, addressing probe design strategies, fabrication issues and providing practical examples of detailed methods for generation of high quality DNA microarray data. Chapters incorporate information on some of the largest providers of microarray, including Affymetrix, Ilumina and Agilent, and their use on a variety of applications. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Covers the use of DNA array from the largest commercial providers Includes practical details to design and produce your own probes and arrays Provides methods for gene expression profiling, SNP mutation analysis, comparative genomic hybridization, resequencing, and ChIP on chip assays INDICE: From the contents Introduction to microarray technology.- Probe design for expression arrays using Oligowiz.- Comparative genomic hybridization:microarray design and data interpretation.- Design of tag SNP whole genome genotyping arrays.- Fabrication of DNA microarray.- Immobilization chemistries.-Fabrication using contact spotter.- RNA preparation and characterization for gene expression studies.- Gene expression analysis using Agilent DNA microarrays.- Target preparation for genotyping specific genes or gene segments.- Genotyping of mutations in the beta-globin gene using allele specific hybridization.- Microarray temperature optimization using hybridization kinetics.- Whole-Genome Genotyping on bead arrays.- Genotyping single nucleotide poymorphosms by multiplex minisequencing using Tag arrays.- Resequencing arrays for diagnostics of respiratory pathogens.- Comparative genomic hybridization: DNA preparation for microarray fabrication.
- ISBN: 978-1-934115-69-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 265
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés