Innovation in biotechnology that combine molecular biology, cell biology, microfabrication and bioinformatics are moving nucleic acid technologies from futuristic possibilities into common laboratory and clinical procedures. They have potential to revolutionize biomedical research and medicine. Three variants of RNA technologies are known, namely antisense oligonucleotides, RNA interference, and ribozymes. In spite of different mechanisms of action, all of them are united by the common principle: an antisense preparation works after binding with RNA-target by forming a duplex. Today all three approaches are intensively used in vivo. RNA mediated gene silencing is a powerful technology to downregulate the expression of targeted genes within the cell. Now RNA interference based therapeutics is to be released. This book provides a state-of-the-art review of the molecular basis as well as applications of RNA based technologies in molecular biology and medicine. RNA mediated gene silencing is a powerfultechnology to downregulate the expression of targeted genes within the cell. Now RNA interference based therapeutics is to be released. This book provides a state-of-the-art review concerning the molecular basis as well as applications of RNA based technologies in molecular biology and medicine. These topics are covered by 19 contributions written by internationally respected experts inthe field of RNA Technologies. INDICE: Preface.- The key features of RNA silencing. Selected strategies for the delivery of siRNA in vitro and in vivo. RNAi suppression and its application. Strategies to prevent siRNA-triggered cellular toxicity. RNAi in malignant brain tumors: relevance to molecular and translational research. SilencingHuntington's disease (HD) gene with RNAi. Application of Dicer-substrate siRNA in pain research. RNAi treatment of HIV-1 infection. Application of RNA Interference to Treat Conditions Associated with Dysregulation of TRPV1. Harnessing RNAi-based Functional Genomics to Unravel the Molecular Complexity Underlying Skin Pigment Variation. mRNA structure and its effects on post-transcriptional gene silencing. Antisense RNA-mediated regulation of the p53 tumor suppressor. Antisense oligonucleotides: insights from preclinical studies and clinicaltrials. What can the new hammerhead ribozyme structures teach us about design? microRNA biogenesis and its impact on RNA interference. MiroRNAs in Epithelial Anti-microbial Immunity. Emerging roles of long noncoding RNAs in gene expression and intracellular organization. Noncoding RNAs as therapeutic targets. Non coding RNAs at H19/IGF2 locus: Role in imprinting, gene expression and associated pathologies.
- ISBN: 978-3-642-12167-8
- Editorial: Springer
- Encuadernacion: Cartoné
- Páginas: 350
- Fecha Publicación: 30/07/2010
- Nº Volúmenes: 1
- Idioma: Inglés