The study of the extracellular matrix has significantly expanded over the past decade. Although thoroughly researched, the complexity and the dynamic nature of the matrix are still under extensive investigation, yielding a growing number of methods of analysis. In Extracellular Matrix Protocols: Second Edition, expert researchers explore this developing field and provide a number of techniques which can be reproduced in most cell biology labs. This edition contains methods that represent cutting edge unique technologies which are well worth exposure to the broad field and may encourage collaboration with expert labs. Chapters include information on molecular biology techniques to study gene expression, biophysical and biochemical methods for the analysis of structure and composition, and cell biology methods for assessment of cell behavior, matrix assembly and tissue engineering applications. A comprehensive protocol bookfeaturing cutting-edge methods and techniques to study molecular, cellular, biochemical, and biophysical aspects of the extracellular matrix Bench-tested in-vivo, ex-vivo and in-vitro methods written by experts who provided detailed step-by-step instructions and trouble-shooting Easy-to-follow techniques that ensure reproducibility and save time and resources INDICE: From the contents Retroviral delivery of ECM genes.- Tissue-Specific KO of ECM Proteins.- Recombinant Collagen Trimers from Insect Cells and Yeast.- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag.- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes.- Preparation of recombinant fibronectin fragments for functional and structural studies.- Quantitative Determination of Collagen Cross-links.- CM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy.- Atomic Force Microscopy Measurements of Intermolecular Binding Forces.- Mass-Mapping of ECM Macromolecules by ScanningTransmission Electron Microscopy.- Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS).- ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy.- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates.
- ISBN: 978-1-58829-984-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 335
- Fecha Publicación: 01/02/2009
- Nº Volúmenes: 1
- Idioma: Inglés