Single-Cell proteomic experiments are difficult to set up and costly to run. Revealing Unchartered Biology with Single Intact Cells: Case Studies explore the path to research success followed by key projects, exploring the role of the techniques, the selection process, what other alternatives were considered, what other paths have lead to dead ends, the detailed protocols followed and how the analysis of generated data allowed the researchers to visualize unchartered biology. Focusing on the research journey that led to the publication of each article, the Editors interviewed the researchers on the use of the Multiplex Single Cell technique and how it helped hone in on the biological quest. The methods used in each case are described in detail, following a standard format to provide consistency across the book. In conclusion to each chapter, the authors review their process critically and provide suggestions of improvement or other techniques that could be employed. Revealing Unchartered Biology with Single Intact Cells: Case Studies is the ideal reference for researchers coming in new to the world of single-cell multiplex techniques. The discussion on failures encountered along the research path provide insights on how to avoid repeating the same errors. The methods as well as lessons learned can be expanded to a wide variety of research objectives. Provides insights into the path to success of key research articles based on Multiplex Single-Cell analysis techniques results Contains detailed method information Discusses strengths and limitations of techniques applied to each research domain covered Includes discussion on the failures encountered along the research path and how to avoid them INDICE: I. Coordinated cellular neighborhoods orchestrate antitumoral immunity at the colorectal cancer invasive front by Schürch et al. 1. Revealing Unchartered Biology 2. Research methods and protocols used in the experiment 3. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research II. Commonly Occurring Cell Subsets in High-Grade Serous Ovarian Tumors Identified by Single-Cell Mass Cytometry by Gonzalez et al. 4. Revealing Unchartered Biology 5. Research methods and protocols used in the experiment 6. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research III. Mass cytometry of Hodgkin lymphoma reveals a CD41 regulatory T-cell-rich and exhausted T-effector microenvironment, by Cader et al. 7. Revealing Unchartered Biology 8. Research methods and protocols used in the experiment 9. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research IV. Bystander CD8+ T cells are abundant and phenotypically distinct in human tumour infiltrates by Simoni et al. 10. Revealing Unchartered Biology 11. Research methods and protocols used in the experiment 12. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research V. High-resolution myogenic lineage mapping by single-cell mass cytometry by Porpiglia et al. 13. Revealing Unchartered Biology 14. Research methods and protocols used in the experiment 15. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research VI. Single-cell mass cytometry reveals distinct populations of brain myeloid cells in mouse neuroinflammation and neurodegeneration models, by Ajami et al. 16. Revealing Unchartered Biology 17. Research methods and protocols used in the experiment 18. Failures encountered along the research path, suggestions of improvement and additional techniques to employ in similar research VII. Conclusion
- ISBN: 978-0-12-822213-3
- Editorial: Academic Press
- Encuadernacion: Rústica
- Páginas: 300
- Fecha Publicación: 01/06/2023
- Nº Volúmenes: 1
- Idioma: Inglés