Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms

Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms enable researchers in Bioscience to visualize cellular phenomena at an unprecedent scale and rate. In Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms the Editors provide a throughout overview of each of the most current techniques both individually and when used in conjunction. Aiming at helping researchers identify the most appropriate method for their biological research question, the book focuses on how to make meaningful use of the tools. In part one the Editors cover CyTof, Multicolor fluorescence cytometry, CODEX multiplexed imaging, MIBI, Imaging Mass Cytometry, Multi resolution cell orientation, and Confocal microscopy. The second part of the book is devoted to the appropriate use of combined techniques such as QPI and Fluorescence microscopy, CyTOF and SCRNA Seq, CyTOP and scATAC Seq, and CyTOF and ATAC See. Part three covers the application of the techniques to biological questions, including chapters on understanding cellular diversity, supporting and rejecting hypothesis, generating hypothesis through data analysis. The final part is dedicated to further the reader's understanding of complex biological systems by identifying new clinical tools, and drug development. Revealing Unchartered Biology with Single Intact Cells: Currently Available Platforms is the ideal reference for researchers willing to understand the variety of Multiplex visualization techniques available for bioscientists and most importantly, how to make better decisions on how to apply them to the biological question of interest. Pharmaceutical science researchers may also benefit from the application of the techniques to identify new possible theraphies and develop new drugs. Covers the most relevant Multiplex techniques applied to Proteomics Provides insights into using the right tool for the biological question of interest Discusses applications of the techniques in several settings Provides an overview of the use of combined techniques INDICE: I. Single Cell imaging techniques 1. CyTOF 2. CODEX Multiplexed Imaging 3. MIBI 4. Imaging Mass Cytometry 5. Multi-resolution cell orientation 6. Confocal microscopy II. Combining techniques 7. QPI and Fluorescence Microscopy 8. CyTOF and scRNA-Seq 9. CyTOF and scATAC-Seq III. Applying techniques to biological questions 10. Understanding cellular diversity in health and disease 11. Interpreting data 12. Supporting/rejecting hypothesis 13. Generating hypothesis through data analysis IV. Furthering our understanding of complex biological systems 14. Identifying a new clinical tools for diagnosis, prognosis and optimal therapeutic selection 15. Drug development applications (asynchronicity of drug responsiveness)

• ISBN: 978-0-12-822209-6
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 300
• Fecha Publicación: 01/06/2023
• Nº Volúmenes: 1
• Idioma: Inglés