RNA Methodologies: A Laboratory Guide for Isolation and Characterization

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike.  This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting from his own personal experience in this subject area.  RNA Methodologies, Sixth Edition, presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. This comprehensive updated edition equips the next generation of scientists with cutting-edge techniques the latest methodologies, and key information for their own laboratory work and study in this ever-changing and exciting field. Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biologyBuilds from basic information on RNA techniques to in-depth protocols for specific applicationsFeatures new chapters on RNA sequencing and RNA in situ hybridizationIncludes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirusComprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics INDICE: 1. RNA and the Cellular Biochemistry Revisited 2. Creating a Ribonuclease-Free Environment 3. Stringency: Conditions That Influence Nucleic Acid Structure 4. RNA Isolation Strategies 5. Isolation of Polyadenylated RNA 6. The Truth About Tissues 7. Going Green: RNA and the Molecular Biology of Plants 8. Quality Control for RNA Preparations 9. cDNA: A Permanent Biochemical Record of the Cell 10. RT-PCR: A Science and an Art Form 11. Quantitative PCR Techniques 12. miRNA and Other Noncoding RNAs 13. RNA Interference and RNA Editing 14. Electrophoresis of RNA 15. Photodocumentation and Image Analysis 16. Northern Analysis 17. Nucleic Acid Probe Technology 18. Quantification of Specific mRNAs by Nuclease Protection 19. Analysis of Nuclear RNA 20. RNA In-situ Hybridization 21. Array Analysis of Gene Expression 22. Nonarray Methods for Global Analysis of Gene Expression 23. Transcriptomes and Bioinformatics 24. RNA-seq: The Premier Transcriptomics Tool 25. RNA Biomarker Discovery and Validation 26. Functional Genomics and Transcript Profiling 27. A Few RNA Success Stories

• ISBN: 978-0-323-90221-2
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 920
• Fecha Publicación: 01/05/2022
• Nº Volúmenes: 1
• Idioma: Inglés