Directed Evolution of Proteins

Aimed at providing researchers and students in academia and industry with an in–depth understanding of directed protein evolution, this advanced textbook enables the design of promising experiments and advanced technologies in diversity generation and high–throughput screening. It contains benchmarking systems and computational tools for comparing the performance of random mutagenesis methods, a section on focused mutant library design and concludes with a look at the challenges, barriers and promising strategies for directed evolution Clearly written and richly illustrated, this book also includes many pedagogical features, such as information boxes summarizing the important information in each chapter. INDICE: DIRECTED PROTEIN EVOLUTION. History and Landmarks in Directed Protein Evolution. Industrial and Academic Impact of Directed Protein Evolution. General Consideration on whether and how to start a Directed Evolution Experiment. Exercises. DIVERSITY GENERATION. Introduction to Sequence Space Generation and Exploration. Classification of Random Mutagenesis Method. Exercises. BENCHMARKING SYSTEMS AND COMPUTATIONAL TOOLS FOR COMPARING THE PERFORMANCE OF RANDOM MUTAGENESIS METHODS. DNA–Based Benchmarking Systems. Protein–Based Benchmarking Systems. Analysis and Performance of Random Mutagenesis Methods. Strategies for Complementary Diversity Generation. Exercises. Computational Tools for Random Diversity Generation. Discussion on Smart Diversity Generation and Applicability for Different Protein Folds and Protein Properties. Exercises. FOCUSED MUTANT LIBRARY DESIGN. Classification of Methods for Single and Multi–Site Directed Mutagenesis. Performance and Comparison. Computational Tools for Focused Mutant Library Design. Discussion on Diversity Generation and Applicability for Specific Protein Properties. Exercises. SCREENING TECHNOLOGIES. General Consideration. Formats, Throughput and the Screening Catastrophe. Microtiter Plate Based Systems. Agar Plate Based Systems. Flow Cytometry Based Systems. Microfluidic Devices Based Systems. In Vitro Translation in Microcompartments/Emulsions. General Screening Platforms. General Lessons on Library Size and Obtained Property Improvements. Exercises. GENERAL LESSONS OF PROTEIN EVOLUTION. The Diversity and Screening Catastrophes. Thermal Stability. Activity Towards Non–Natural and Natural Substrates. Selectivity & One Catalyst/Many Substrate Concept. Substrate/Product Concentration. Solvent Resistance (Organic Solvents/Ionic Liquids). pH–Resistance. Salt and Detergent Resistance. Alternative Cofactor Systems. Summary and Principles for Individual Property Improvements. Exercises. CHALLENGES, BARRIERS AND PROMISING STRATEGIES FOR DIRECTED EVOLUTION. Diversity Generation. Computational Simulation. Throughput Technologies and Selection Systems. Natural Evolution vs Directed Evolution. Exercises

• ISBN: 978-3-527-32369-2
• Editorial: Wiley VCH
• Encuadernacion: Cartoné
• Páginas: 400
• Fecha Publicación: 07/06/2018
• Nº Volúmenes: 1
• Idioma: Inglés