MCB: CAR T Cells: Development, Characterization and Applications

MCB: CAR T Cells: Development, Characterization and Applications, Volume 167 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy, Determination of the Biodistribution of Chimeric Antigen Receptor-Modified T Cells against CD19 in NSG Mice,  Generation of CAR-T cells using lentiviral vectors, Generation of CAR T-cells using ?-retroviral vector, Flow cytometry detection and quantification of CAR T cells into solid tumors, Evaluation of CAR-T Cell Cytotoxicity: Real-Time Impedance-Based Analysis, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Cell Biology series Includes the latest information on the topic of development, characterization and applications in CAR T Cells INDICE: 1. High-efficiency of genetic modification using CRISPR/Cpf1 system for engineered CAR T-cell therapy Renpeng Ding, Cheng-Chi Chao and Qianqian Gao 2. Determination of the biodistribution of chimeric antigen receptor-modified T cells against CD19 in NSG mice Hairuo Wen, Ying Huang, Tiantian Hou, Junzhi Wang and Yan Huo 3. Generation of CAR-T cells using lentiviral vectors Mansour Poorebrahim, Isaac Quiros-Fernandez, Elham Fakhr and Angel Cid-Arregui 4. Phenotyping of CAR T cells Sanjay Rathod 5. Evaluation of CAR-T cell cytotoxicity: Real-time impedance-based analysis Amanda N. Lisby, Robert D. Carlson, Trevor R. Baybutt, Megan Weindorfer and Adam E. Snook 6. Flow cytometry detection and quantification of CAR T cells into solid tumors Nicholas J.A. Tokarew, Javier Suárez Gosálvez, Alessia Nottebrock, Daria Briukhovestka, Stefan Endres, Bruno L. Cadilha and Sebastian Kobold 7. Generation and functional characterization of CAR exosomes Chuqi Wang, Wenyan Fu, Changhai Lei and Shi Hu 8. Preclinical testing of CAR T cells in zebrafish xenografts Sarah Grissenberger, Benjamin Salzer, Susana Pascoal, Andrea Wenninger-Weinzierl, Manfred Lehner and Martin Distel 9. In vivo experimental mouse model to test CD19 CAR T cells generated with different methods Anne Fåne, Marit Renée Myhre, Else Marit Inderberg and Sébastien Wälchli 10. Non-invasive fluorescence imaging for tracking immune cells in preclinical models of immunotherapy Marcelo de Souza Fernandes Pereira, Aarohi Thakkar and Dean Anthony Lee 11. Generation of CAR T-cells using ?-retroviral vector Norihiro Watanabe and Mary Kathryn McKenna 12. Methods to monitor in vivo expansion and efficacy of CAR-T cells in preclinical models Mohammad Alhomoud, Jeremie Martinet, Mayumi Sugita, Alexandra Gomez-Arteaga and Monica L. Guzman 13. Strategies for manufacturing cell therapy products aligned with patient needs Prajna Guha and Steven C. Katz

• ISBN: 978-0-323-85501-3
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 242
• Fecha Publicación: 15/02/2022
• Nº Volúmenes: 1
• Idioma: Inglés