Molecular Characterization of Autophagic Responses Part A

Molecular Characterization of Autophagic Responses, Part A, presents a collection of methods for the qualitative and quantitative evaluation of virtually all the morphological, biochemical, and functional manifestations of autophagy, in vitro, ex vivo and in vivo, in organisms as distant as yeast and man. Autophagy is an evolutionarily conserved mechanism for the lysosomal degradation of superfluous or dangerous cytoplasmic entities, and plays a critical role in the preservation of cellular and organismal homeostasis. Monitoring the biochemical processes that accompany autophagy is fundamental for understanding whether autophagic responses are efficient or dysfunctional. Offers a detailed overview of the protocols used to study autophagy and various aspects of autophagic responsesWritten in an accessible style by renowned experts in the field INDICE: Renilla luciferase-based reporter assay for measuring autophagic fluxMarja JäätteläAssessing the catalytic activity of transglutaminases in the context of autophagic responsesMauro PiacentiniMethods to measure lysosomal pH in the course of autophagySergio GrinsteinLong-lived protein degradation during autophagyPatrice CodognoProteomic profiling of de novo protein synthesis in starvation-induced autophagy using bio-orthogonal noncanonical amino acid taggingHan-Ming ShenMethods to monitor TFEB activation during autophagy Andrea BallabioApplication of CRISPR/Cas9 to autophagy researchKevin M. RyanA Molecular Reporter For Monitoring Autophagic Flux In Nervous System In Vivo Claudio HetzMagnetic Resonance spectroscopy to study glycolytic metabolism during autophagyYuen-Li ChungAssessment of glycolytic flux and mitochondrial respiration in the course of autophagic responsesLorenzo GalluzziMethods to assess mitochondrial morphology in mammalian cells mounting autophagic or mitophagic responsesPaolo PintonFluorescent reporters to characterize mitophagy in mammalian systemsQuan ChenCytofluorometric assessment of mitophagic flux in mammalian cells and tissuesPatricia BoyaAutomated analysis of fluorescence co-localization - Application to mitophagyOliver KeppAssays to monitor lysophagyWei Yuan YangKinetics of protein aggregates disposal by aggrephagyEsther WongMethods to study chaperone-mediated autophagyEsperanza AriasQuantitative assay of macroautophagy using Pho8-60 assay and GFP-cleavage assay in yeastTakeshi NodaMonitoring the formation of autophagosomal precursor structures in yeast Saccharomyces cerevisiaeFulvio ReggioriMethods to assess autophagy-dependent lifespan extension in yeastFrank MadeoAssessing intracellular lipid turnover by lipophagy in S. cerevisiaeSepp D. KohlweinAssays to monitor pexophagy in yeastSuresh SubramaniMethods to study autophagic responses in C. elegansNektarios TavernarakisCharacterization of autophagic responses in D. melanogasterDonna DentonMethods to study autophagy in ZebrafishTibor VellaiBiochemical methods to monitor autophagic responses in plantsDiane C. BasshamUsing photoconvertible and extractable fluorescent proteins to study autophagy in plantsKen Matsuoka

• ISBN: 978-0-12-809675-8
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 01/03/2017
• Nº Volúmenes: 1
• Idioma: Inglés