Tumor Immunology and Immunotherapy - Cellular Methods Part A

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Contains the authority of authors who are leaders in their fieldProvides a comprehensive source on new methods and research in enzymology INDICE: 1. Detection of intracellular cytokine production by T cells with flow cytometry Leisha A. Emens 2. High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimers George Coukos 3. In vitro assays for effector T cell functions and activity of immunostimulatory antibodies Roberta Zappasodi 4. Ex vivo energetic profiling of tumor cells and T cells from mouse models and human samples Greg M. Delgoffe 5. A cytofluorimetric assay to evaluate T cell polyfunctionality Paola Nisticò 6. Assessment of memory formation by metabolically engineered antigen-specific CD8+ T cells Pedro Romero 7. Functional characterization of tumor antigen-specific T-cells isolated from the Tumor microenvironment of Sleeping Beauty induced murine glioma models Maria G. Castro 8. Methods to edit T cells for cancer immunotherapy Laurie Menger 9. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancer Enrico Lugli 10. Reverse immunology: from a peptide sequence to a tumor-killing T-cell clone Pierre van der Bruggen 11. Generation of TCR-engineered reference cell samples to control T-cell assay performance Sjoerd van der Burg 12. Optimization of in vitro expansion of tumor-reactive human gamma/delta T cells Dietrich Kabelitz 13. CFSE dilution to study human T and NK cell proliferation in vitro Francisco Borrego 14. Rapid isolation and enrichment of mouse NK cells for experimental purposes Ignacio Melero 15. Rapid, high-yield isolation of human NK cells from the peripheral blood Jitka Fucikova 16. Dyanmic two-dimensional evaluation of NK cell mediated lysis Anne Caignard 17. Rapid, high-yield isolation of mouse ILCs from mucosal surfaces Arthur Mortha 18. Identification of innate lymphoid cells in mouse models Pedro Romero 19. A cytofluorometric assay to evaluate intracellular cytokine production by NK cells Alejandro López-Soto 20. Applications of microfluidic devices in advancing NK-cell migration studies Sam K.P. Kung 21. Complementary approaches to study NKT cells in cancer Jay A. Berzofsky 22. A highly sensitive bimolecular luminescence complementation (BiLC) reporter system for realtime measurement of IFN signaling Xiao-Feng Qin 23. Assessment of IFN? and granzyme B production by in sitro? technology Ilio Vitale 24. Assessment of IFNy responsiveness of patient-derived xenografts Katerina Politi 25. Mixed lymphocytes reaction assay in drug discovery for immune checkpoint blockades Rogze O. Lu 26. Real-time cell analysis (RTCA) to measure CTL activity against adherent tumor cells in vitro Dietrich Kabelitz 27. Analysis of cancer cell-intrinsic immune regulation in response to CTL attack Daniel E. Speiser 28. A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicity Santos Manes 29. Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay Alejandro López-Soto 30. 51Cr-release to monitor NK cell cytotoxicity Ralf Dressel

• ISBN: 978-0-12-818673-2
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 15/01/2020
• Nº Volúmenes: 1
• Idioma: Inglés