The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notessection which shares tips on troubleshooting and avoiding known pitfalls. Offers cutting-edge methods for cancer gene profiling Includes methods for gene expression profiling and array CGH, as well as protein analysis Provides information about collecting clinical samples and their use in microdissection INDICE: From the contents Organizational Issues in Providing High Quality Human Tissues and Clinical Information for the Support of Biomedical Research.- Manual microdissection.- Laser Microdissection.- Tissue Microarrays.- A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function.- Mining EST libraries for cancer associated genes.- Automated fluorescent differential display (FDD) for cancer gene profiling.- Manual microdissection combined with aRNA-longSAGE for the analysis of limited cell numbers.-Quantitative DNA Methylation Profiling on a High Density Oligonucleotide Microarray.- SNP analysis to associate cancer risk.- Application of proteomics in cancer gene profiling: 2D-DIGE.- Search for and Identification of Novel Tumor associated Autoantigens.- Integrative Oncogenomic Analysis Of Microarray Data In HematologicMalignancies.- Cancer gene profiling in pancreatic cancer.- Cancer Gene Profiling in Prostate Cancer.- Cancer Gene Profiling for Response Prediction.
- ISBN: 978-1-934115-76-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 385
- Fecha Publicación: 01/09/2009
- Nº Volúmenes: 1
- Idioma: Inglés