Measurement and Analysis of Kinetic Isotope Effects

Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Written by leading authorities in the field of enzymologyCovers the experimental analysis of enzyme mechanism using isotope effects INDICE: Measurement of enzyme binding isotope effectsVern SchrammChemical ligation and isotope labeling to locate dynamic effectsRudolf AllemanMeasurement of heavy enzyme isotope effectsAmnon KohenExtracting kinetic isotope effects from a global analysis of reaction progress curvesSam HayKIE of metabolic flux and enzymes.Guillaume TcherkezSolvent and Primary KIE on Flavin Enzymes.Paul FitzpatrickThe Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DODJohn Richard18-O isotope effects on oxygenasesJudith KlinmanChlorine isotope effects on dehalogenasesPiotr PanethChlorine isotope effects (w/Paneth)Agnieszka Dybala-DefratykaKinetic Deuterium Isotope Effects in Cytochrome P450 ReactionsF. Peter GuengerichUse of Isotopes and Isotope Effects for Investigations of Diiron Oxygenase MechanismsJohn LipscombCharacterization of substrate, co-substrate, and product isotope effects associated with enzymatic oxygenations using gas- and liquid chromatography/isotope ratio mass spectrometryThomas HofstetterNMR titrations to determine equilibrium isotope effectsCharles PerrinNMR to characterize the nonstatistical distribution of stable isotopes in metabolismRichard RobinsApplications of isotope ratio mass spectrometry in sports drug testing: accounting for isotope fractionation in analysis of biological samplesThomas PiperWhole molecule mass spectrometry to measure KIEs on RNA 2'O-transphosphorylation reactionsMike HarrisIsotope Effects on the Concentration of Steady State IntermediatesHung-Wen Liu and Mark Ruszczycky Headspace analysis of CO2 to measure carbon kinetic isotope effects in decarboxylation reactionsRon Kluger32P/33P remote label methods to measure RNA kinetic isotope effectsJoseph PiccirilliIsotope effects and MS analysis of pseudouridine synthase enzymesEugene MuellerNucleophile isotope effects on glycosyl transfer reactionsAndrew James Bennet

• ISBN: 978-0-12-812273-0
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 10/09/2017
• Nº Volúmenes: 1
• Idioma: Inglés