Handbook of RNA Biochemistry

The second edition of a highly acclaimed handbook and ready reference. Unmatched in its breadth and quality, around 100 specialists from all over the world share their up–to–date expertise and experiences, including hundreds of protocols, complete with explanations, and hitherto unpublished troubleshooting hints. They cover all modern techniques for the handling, analysis and modification of RNAs and their complexes with proteins. Throughout, they bear the practising bench scientist in mind, providing quick and reliable access to a plethora of solutions for practical questions of RNA research, ranging from simple to highly complex. This broad scope allows the treatment of specialized methods side by side with basic biochemical techniques, making the book a real treasure trove for every researcher experimenting with RNA. INDICE: RNA SYNTHESIS.Enyzmatic synthesis, ligation and modification.Chemical synthesis.NEW: RNA detection and quantitation..STRUCTURE DETERMINATION.Sequence determination.NEW: Probing in vitro and in vivo.Footprinting techniques.Nucleotide analog interference mapping.Identification of interacting proteins and nucleic acids.Spectroscopic studies of RNA structure.Atomic structure determination.NEW: Functional assays.NEW: Mass spectrometry of RNA.NEW: Folding and unfolding of single RNA molecules..RNA GENOMICS & BIOINFORMATICS.Phylogenetic analysis.Structure prediction.Modeling of RNA structures.Molecular dynamics simulation.NEW: Searching and predicting small RNAs.NEW: RNA mining and RNomics in eukaryotes.NEW: Exon arrays..ANALYSIS OF RNA–PROTEIN INTERACTION.Isolation and purification of RNA–binding proteins.Sedimentation analysis of RNP complexes.Protein–RNA crosslinking.Immunoaffinity purification.NEW: TAP–tag affinity purification.Three–hybrid analysis to identify RNA–binding proteins..SELEX.In vitro selection of ribozymes and aptamers.In vivo SELEX.In vitro selection against small targets.NEW: Genomic SELEX..RNA INTERFERENCE.NEW: Chemical modification of siRNA.NEW: siRNA design and delivery.NEW: RNAi in mammalian cells..(partial contents).

• ISBN: 978-3-527-32776-8
• Editorial: Wiley VCH
• Encuadernacion: Rústica
• Páginas: 1368
• Fecha Publicación: 20/05/2015
• Nº Volúmenes: 1
• Idioma: Inglés