Transgenic mouse methods and protocols

Transgenic mouse methods and protocols

Hofker, Marten H.
Deursen, Jan van

109,15 €(IVA inc.)

The generation of genetically modified mice is absolutely crucial to gene function studies today, primarily because mice are genetically similar to man andbecause gene function studies in mice are in the context of a whole organism,making them particularly useful. In Transgenic Mouse Methods and Protocols, Second Edition, expert research explore current advances in the field through detailed laboratory protocols. Chapters provide a general introduction outlining how to deal with mice and how to generate transgenic mouse models, explore the generation of conditional and induced knockout and transgenic mice, and offer alternative routes to studying gene function in mice. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and state of the art, Transgenic Mouse Methods and Protocols, second Edition is the ideal guide for all researchers interested in the latest information about the production and analysis of transgenic and knockoutmice. Fully updated from the first edition to offer cutting-edge methods for generation of transgenic mouse models Provides descriptions of how to generateconventional knockout and transgenic mice Includes detailed protocols for vector preparation of different kinds of mouse models Methods to control expression patterns of the transgenes are provided INDICE: Introduction: Strategies for developing genetically modified mice.- Genetic Modification of the Mouse; General Technology: Pronuclear and Blastocyst Injection.- Generation of chimeras by aggregation of embryonic stem cellswith diploid or tetraploid mouse embryos.- Cryopreservation of mouse spermatozoa and In vitro fertilization.- Autopsy and histological analysis of the transgenic mouse.- Transgene design.- Inducible Transgenic Mouse Models.- Lentiviral transgenesis.- Transgenesis in Mouse Embryonic Stem Cells.- Engineering BACReporter Gene Constructs for Mouse Transgenesis.- Targeting Vector Construction Through Recombineering.- Generating Conditional Knockout Mice.- Hypomorphicmice.- MICER Targeting Vectors for Manipulating the Mouse Genome.- Knockin approaches.- Generation of a series of knockin alleles using RMCE in ES cells.- Selection of targeted mutants from a library of randomly mutagenized ES cells .- Generation of genetically modified rodents using random ENU mutagenesis.- Bone marrow transplantations to study gene function in hematopoietic cells.- Adenovirus Mediated Gene Transfer.

  • ISBN: 978-1-60761-973-4
  • Editorial: Humana
  • Encuadernacion: Cartoné
  • Páginas: 322
  • Fecha Publicación: 29/10/2010
  • Nº Volúmenes: 1
  • Idioma: Inglés