The knowledge gained by studying germline stem cells, the source of human andanimal reproduction, may find immediate application in preserving endangered wildlife, managing commercial livestock, overcoming fertility problems in humans, and treating testicular and ovary tumors. In Germline Stem Cells, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. Divided into two parts, the volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells. Comprehensive and cutting-edge, Germline Stem Cells is the perfect reference for scientists exploring this particular exciting and important subdivision in the fast-paced field of stem cell research. Readily reproducible protocols for the manipulation of germlinestem cells in model organisms. State of the art methods optimized by leading experts in germline stem cells. Identification and regulation of germline stemcell in model organisms. Current techniques used in in vitro culture and application in germline stem cells. INDICE: From the contents The Development of Germline Stem Cells in Drosophila. Analysis of the C. elegans Germline Stem Cell Region. ImmunohistologicalTechniques for Studying the Drosophila Male Germline Stem Cell. Genetic ToolsUsed for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells. Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (the milkweed bug Oncopeltus fasciatus). High Resolution Light Microscopic Characterization of Spermatogonia. Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sectionsfrom Primate Testes. Epigenetic Control in Male Germ Cells. GDNF Maintains Mouse Spermatogonial Stem Cells in vivo and in vitro. Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts. Spermatogonial Stem Cell Transplantation, Testicular Function and Restoration of Male Fertility in Mice.
- ISBN: 978-1-60327-213-1
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 295
- Fecha Publicación: 01/05/2008
- Nº Volúmenes: 1
- Idioma: Inglés