Protocols in Biochemistry and Clinical Biochemistry

Protocols in Biochemistry and Clinical Biochemistry

Jain, Buddhi Prakash
Goswami, Shyamal K.
Pandey, Shweta

116,48 €(IVA inc.)

Protocols in Biochemistry and Clinical Biochemistry offers clear, applied instruction in fundamental biochemistry methods and protocols, from buffer preparation to nucleic acid purification; protein, lipid, carbohydrate, and enzyme testing; and clinical testing of vitamins, glucose, and cholesterol levels, among other diagnostics. Each protocol is illustrated with step-by-step instructions, labelled diagrams, and color images, as well as a thorough overview of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshooting. Offers comprehensive coverage of biochemistry protocols for research and clinical laboratoriesIncludes full listings and discussion of materials and equipment, precursor techniques, safety considerations and standards, analysis and statistics, alternative methods, and troubleshootingFeatures clear, step-by-step protocols instructions with color diagrams and images INDICE: Unit I: Buffer, solution and pH 1. Preparation of normal and molar solution 2. Buffer preparation 3. Determination of pKa, pH Unit II: Nucleic Acids 1. Isolation and Purification of DNA 2. Isolation and Purification of RNA 3. Purification of Nucleic Acid 4. Quantitative estimation of Nucleic acid 5. Agarose gel electrophoresis for separation of nucleic acid Unit III: Carbohydrate and Lipids 1. Qualitative test for carbohydrate (Benedict, Molisch etc) 2. Qualitative test for Lipids 3. Thin liquid chromatography (TLC for separation and identification of carbohydrate and lipids Unit III: Proteins 1. Isolation of Protein 2. Qualitative test of Protein ( Biuret test, Xanthoproteic test, Ninhydrin test etc) 3. Estimation of Protein (Bradford, Lowry etc) 4. Paper Chromatography for separation and identification of amino acid 5. Ion exchange chromatography for separation of amino acid 6. Protein purification 7. SDS PAGE analysis of proteins 8. Western blotting for protein analysis 9. Isoelectric pH determination Unit IV: Enzymes 1. Enzyme kinetics 2. Study of the activity of salivary amylase and the effect of pH, temperature, salts on its activity 3. Enzymatic activity of Trypsin and Lipase 4. Enzyme purification 5. Determination of Km, Vmax and Ki Unit V: Clinical test 1. Acid and Alkaline phosphatase activity 2. SGPT and SGOT test 3. Estimation of Urea, Uric acid and Creatinine 4. Estimation of Glucose 5. Estimation of Cholesterol 6. Estimation of Vitamins 7. Glucose tolerance test 8. Estimation of Ca2+ and T4 in serum 9. Pregnancy test by HCG hormone 10. Estimation of Glycosylated haemoglobin

  • ISBN: 978-0-12-822007-8
  • Editorial: Academic Press
  • Encuadernacion: Rústica
  • Páginas: 200
  • Fecha Publicación: 01/08/2020
  • Nº Volúmenes: 1
  • Idioma: Inglés