Including an overview of progress made in the field over the past decade, Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. This one–stop reference for consistent methods and reliable tools spans the entire assay work flow, from isolation or generation of neural stem cells to characterization, manipulation and final application of NSCs in disease paradigms, such as Parkinson?s disease, multiple sclerosis, and ALS. This is an excellent source of information for academic, pharmaceutic and biotechnology researchers. INDICE: Foreword: Neural Stem Cell Methods – An Overview Part 1: Isolation of Neural stem cell and progenitor from tissue Overview of Neural Stem cells and progenitor cells from tissue NSC/NPC isolation from fetal mouse/Rat/human (neurosphere) OPC isolation from fetal brain Neural induction and Generation of NSC from pluripotent cells (hESC/iPSC) Part 2: Derivation of Neural stem and progenitor from PSC NSC/NPC Directed differentiation into Dopaminergic neurons Directed differentiation into Motor neurons Directed differentiation into GABAergic neurons Directed differentiation into Astrocytes Directed differentiation into Oligodendrocytes Part 3: Expansion and cryopreservation Culture methods of adult/fetal derived NSC from rodents Culture methods for the expansion of human NSC and NPC Primary neural cultures from Rat/mouse/human Xeno–Free Culture Expansion of hNSC Cryopreservation of neural stem and progenitor cells Cryopreservation of Primary neural cells Part 4: Cellular Characterization tools NSC marker analysis by immunocytochemistry Surface Antibody analysis by FACS Bead–based separation/antibody panning for A2B5 cells Neural Cell viability Assay Electrophysiology of neurons Transplantation of NSC/NPC in animal models of disease Part 5: Molecular characterization tools RNA isolation and cDNA preparation Multiplex end–point PCR PLA assay Micro RNA Analysis Transduction methods for NSC Gene expression analysis
- ISBN: 978-1-118-30825-7
- Editorial: Wiley–Blackwell
- Encuadernacion: Cartoné
- Páginas: 250
- Fecha Publicación: 09/09/2014
- Nº Volúmenes: 1
- Idioma: Inglés