Methods in Enzymology, Volume 644 continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Methods for Studying RNA condensation/granules in vitro, RNA Dynamics in Intracellular Condensates, Methods for Viscoelastic Characterization of Liquid and Gel Condensates, Incorporating Proteins into Complex Coacervates, Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes, Preparation of and Solute Partitioning in Multiphase Coacervates, Reversible photocontrol of DNA coacervation, Enzymatic Control over Coacervation, and much more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Enzymology series INDICE: 1. Methods for Studying RNA condensation/granules in vitro Roy Parker 2. RNA Dynamics in Intracellular Condensates Geraldine Seydoux 3. Methods for Viscoelastic Characterization of Liquid and Gel Condensates Priya Banerjee 4. Incorporating Proteins into Complex Coacervates Sarah Perry 5. Methods for Study of Liquid-Liquid Phase Coexistence in Proximity to Lipid Membranes Michael Rosen 6. Preparation of and Solute Partitioning in Multiphase Coacervates Christine Keating 7. Reversible photocontrol of DNA coacervation Nicolas Martin 8. Enzymatic Control over Coacervation Evan Spruijt 9. Reconstituting Bacterial Cytoskeletal Assembly in Crowded, Phase-Separated Media Germán Rivas 10. Copolymer-driven stabilisation of complex coacervate microdroplets Jan C. M. van Hest 11. Measuring Activity and Structure of Nucleic Acid Enzymes in Complex Coacervates via Gel Electrophoresis Philip C. Bevilacqua 12. SAXS Methods for Studying Macromolecular Phase Separation Matthew Tirrell 13. Protein Engineering for Control over Membraneless Organelles in Artificial Cells Dan Hammer 14. Ultra-small neutron scattering of coacervates Vivek M. Prabhu 15. Small angle X-ray scattering experiments of monodisperse samples close to the solubility limit Tanja Mittag 16. Theory of biological phase separation Jeetain Mittal
- ISBN: 978-0-12-821159-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 412
- Fecha Publicación: 15/10/2020
- Nº Volúmenes: 1
- Idioma: Inglés