Only in recent times has the possibility of growing and implanting replacement teeth, made from one’s own cells, moved into the realm of realistic possibilities; however, the molecular and cellular mechanisms of tooth development must be studied in a range of vertebrates, from zebrafish to mice, so that evolutionarily conserved network kernels, which will define the cellular states of generic vertebrate tooth development, can be recognized. In Odontogenesis: Methods and Protocols, experts in the field examine techniques to approach this burgeoning field. This detailed volume includes chapters on the detection of “tooth development” gene expression, both at the RNA and protein level, currentapproaches to the manipulation of gene expression levels and subsequent analysis of tooth phenotypes, as well as chapters concerning current efforts to getliving tooth implants working without waiting for a full understanding of thedevelopmental pathways at the molecular level. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.Practical and easy to use, Odontogenesis: Methods and Protocols aims to help researchers move forward toward the ultimate goal of getting a “bioengineered tooth” into the patient’s mouth. INDICE: 1. Histological Analysis of the Embryonic and Adult Tooth Atsushi Ohazama 2. Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, and Chrissa Kioussi 3. Determination of Gene Expression Patterns by In Situ Hybridization in Sections Kateryna Kyrylkova, Sergiy Kyryachenko, Chrissa Kioussi, and Mark Leid 4. Immunohistochemistry and Detection of Proliferating Cells by BrdU Sergiy Kyryachenko, Kateryna Kyrylkova, Mark Leid, and Chrissa Kioussi 5. Detection of Apoptosis by TUNEL Assay Kateryna Kyrylkova, Sergiy Kyryachenko, Mark Leid, and Chrissa Kioussi 6. Use of siRNA in Dental Tissue-Derived Cell Cultures: Integrin Knockdown in Fibroblasts Malgorzata M. Barczyk, Donald Gullberg, and Anne Isine Bolstad 7. Organ Cultures and Kidney-Capsule Grafting of Tooth Germs Keishi Otsu, Naoki Fujiwara, and Hidemitsu Harada 8. Evaluation of Skull and Tooth Morphology and Mineralization Using High-Resolution X-Ray Tomography Brian K. Bay 9. Electron Microscopy Hans U. Luder and Margrit Amstad-Jossi 10. Deoxyoligonucleotide Microarrays for Gene Expression Profiling in Murine Tooth Germs Harald Osmundsen, Anne-Marthe Jevnaker, and Maria A. Landin 11. Lineage Differentiation of Mesenchymal Stem Cells from Dental Pulp, Apical Papilla, and Periodontal Ligament Kentaro Akiyama, Chider Chen, Stan Gronthos, and Songtao Shi 12. In Vivo Transplantation and Tooth Repair Shuhei Tsuchiya and Masaki Honda 13. Methods to Validate Tooth-Supporting Regenerative Therapies Miguel Padial-Molina, Julie T. Marchesan, Andrei D. Taut, Qiming Jin, William V. Giannobile, and Hector F. Rios 14. Generation of a Bioengineered Tooth by Using a Three-Dimensional Cell Manipulation Method (Organ Germ Method) Masamitsu Oshima, Miho Ogawa, Masato Yasukawa, and Takashi Tsuji 15. In Vitro Studies on Odontogenic Tumors Javier Catón, Thimios A. Mitsiadis, and Peter R. Morgan 16. Whole Mount Immunohistochemistry and In Situ Hybridization of Larval and Adult Zebrafish Dental Tissues Barbara Verstraeten, Ellen Sanders, and Ann Huysseune
- ISBN: 978-1-61779-859-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Fecha Publicación: 31/05/2012
- Nº Volúmenes: 1
- Idioma: Inglés