Stem Cell Laboratory Techniques: A Guide for Researchers and Students

Stem Cell Laboratory Techniques: A Guide for Researchers and Students introduces the reader to stem cell culture, handling techniques and versatile applications used by researchers. Sections introduce stem cells, including definitions, types and basic use of stem cells in biomedical science research. The book explains laboratory procedures and techniques ranging from the extraction of stem cells from animals, cell seeding and culture, harvest and maintenance of stem cells, stem cell characterization, accurate recording, quality control, and more. In addition, it guides researchers on topics such as transcriptome analysis, proliferation study analysis, and microphysiological study. Final sections cover useful and recent applications in stem cells, such as gene editing techniques and the preparation of stem cells for in vitro study, as well as stem cell lab design and equipment used in the lab. Lastly, human and animal research ethics are discussed. Introduces readers to the stem cell culture and moves to handling techniques and versatile applications Includes coverage of gene editing techniques for stem cells and stem cells for in vitro study Presents stem cell lab design and equipment used in the lab INDICE: 1. Introduction: Stem Cell Definition, Types of Stem Cells, Use and Application of Stem Cell2. Extraction of Stem Cells from Animal Models3. Harvest and Culture of Stem Cells4. Establishment of Amniotic fluid Stem Cell5. Stem Cell Characterization6. Good Recording and Quality Control7. Differentiation Techniques for Confirmation Studies8. Transcriptomic Analysis9. Proliferation Study and Analysis10. Single Cell Isolation Methods and their Impact on Stem Cell Study11. Gene Editing Techniques for Stem Cell12. Preparation of Cells for in vitro study13. Stem Cell Lab Design and Equipment14. Human and Animal Research Ethics15. Organoids

• ISBN: 978-0-12-823729-8
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 250
• Fecha Publicación: 01/04/2023
• Nº Volúmenes: 1
• Idioma: Inglés