Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessarymaterials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Includes 52 different protein transfer and detection techniques Gives a brief overview of 35 additional protein transfer and detection methodologies Provides a history of protein transfer development and an overview of membrane proteomics Outlines inexpensive protein blotting and detection protocols useful for large college courses INDICE: From the contents Origins of protein blotting.- Western Blotting: Remembrance of Things Past.-Introduction to protein blotting.- Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis.- Diffusion blotting for rapid production of multiple identical imprintsfrom sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support.- Tryptic peptide purification using polyvinylidene difluoride membrane for matrix assisted laser desorption ionization time of flight mass spectrometry.- Affinity immunoblotting for analysis of antibody clonotype distribution ina lupus patient developing anti-Ro 60 over time.- Non-electrophoretic bi-directional transfer of a single SDS-PAGE gel with multiple antigens to obtain twelve immunoblots.- Slice blotting.- Localizing Proteins by Tissue Printing.- Dot-Immunobinding Assay (Dot-Iba).- Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes after Agarose-gel Electrophoresis Separation.
- ISBN: 978-1-934115-73-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 590
- Fecha Publicación: 01/06/2009
- Nº Volúmenes: 1
- Idioma: Inglés