With the completion of human genome sequencing, the field of proteomics has grown vital to biomedical research. Among the array of protocols developed to study the human proteome, many protocols examine proteins derived specifically from cell lines, body fluids or human tissues - not necessarily compatible with the full range of protein sources commonly used in the laboratory. Tissue Proteomics constitutes in one volume useful and innovative protocols developed specifically for the proteomic profiling of human tissues. A unique resource for researchers, Tissue Proteomics provides high-throughput gel-based techniques, microarrays and a number of other methods used in proteomic research, a field still in its early stages. This important book will prove indispensable to investigators of biomarker discovery and therapeutic response profiling, as well as those forging new paths in the fields of theranostics and personalized medicine. Provides time tested, reproducible laboratory methods and protocols authored by experts in the field, including tips and hints. Presents the most upto date and useful proteomic techniques for profiling human tissue. Provides indispensable protocols for biomarker discovery, therapeutic response profiling, and high throughput based techniques. INDICE: From the contents Capabilities using 2-D DIGE in Proteomics Research: The New Gold Standard for Two-dimensional Gel Electrophoresis.- Protein Carbohydrate Analysis: Gel-based Staining, Liquid Chromatography, Mass Spectrometry and Microarray Screening.- Surface-Enhanced Laser Desorption/Ionization Mass Spectrometry (SELDI-MS) for Protein and Peptide Profiling of Body Fluids.- A Novel Approach using MALDI-TOF/TOF Mass Spectrometry and Prestructured Sample Supports (AnchorChip technology) for Proteomic Profiling and Protein Identification.- Automated Laser Capture Microdissection for Tissue Proteomics.- Tissue Microarrays: An overview.- Frozen Protein Arrays.- Reverse Phase Protein Microarrays for Theranostics and Patient Tailored Therapy.- Antibody Arrays for Determination of Relative Protein Abundances.- SH2 Domain Based Tyrosine Phosphorylation Array.- Immunoregulomics: a serum autoantibody-based platform for transcription factor profiling.
- ISBN: 978-1-58829-679-5
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 300
- Fecha Publicación: 01/04/2008
- Nº Volúmenes: 1
- Idioma: Inglés