Bioanalytics: Analytical Methods and Concepts in Biochemistry and Molecular Biology

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross–references in order to highlight the many connections between different techniques. The book provides a bird´s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: – Purification and determination of proteins – Measuring enzymatic activity – Microcalorimetry – Immunoassays, affinity chromatography and other immunological methods – Cross–linking, cleavage, and chemical modification of proteins – Light microscopy, electron microscopy and atomic force microscopy – Chromatographic and electrophoretic techniques – Protein sequence and composition analysis – Mass spectrometry methods – Measuring protein–protein interactions – Biosensors – NMR and EPR of biomolecules – Electron microscopy and X–ray structure analysis – Carbohydrate and lipid analysis – Analysis of posttranslational modifications – Isolation and determination of nucleic acids – DNA hybridization techniques – Polymerase chain reaction techniques – DNA sequence and epigenetic modification analysis – Analysis of protein–nucleic acid interactions – Analysis of sequence data – Proteomics, metabolomics, peptidomics and toponomics – Chemical biology INDICE: Preface XV .Introduction XIX .Part I Protein Analytics 1 .1 Protein Purification 3 .2 Protein determination 23 .3 Enzyme Activity Testing 35 .4 Microcalorimetry 47 .5 Immunological Techniques 63 .6 Chemical Modification of Proteins and Protein Complexes 107 .7 Spectroscopy 131 .8 Light Microscopy Techniques Imaging 181 .9 Cleavage of Proteins 207 .10 Chromatographic Separation Methods 219 .11 Electrophoretic Techniques 243 .12 Capillary Electrophoresis 275 .13 Amino Acid Analysis 301 .14 Protein Sequence Analysis 313 .15 Mass Spectrometry 329 .16 Protein Protein Interactions 381 .17 Biosensors 419 .Part II 3D Structure Determination 431 .18 Magnetic Resonance Spectroscopy of Biomolecules 433 .19 Electron Microscopy 485 .20 Atomic Force Microscopy 519 .21 X–Ray Structure Analysis 529 .Part III Peptides, Carbohydrates, and Lipids 553 .22 Analytics of Synthetic Peptides 555 .23 Carbohydrate Analysis 571 .24 Lipid Analysis 613 .25 Analysis of Post–translational Modifications: Phosphorylation and Acetylation of Proteins 645 .Part IV Nucleic Acid Analytics 663 .26 Isolation and Purification of Nucleic Acids 665 .27 Analysis of Nucleic Acids 681 .28 Techniques for the Hybridization and Detection of Nucleic Acids 719 .29 Polymerase Chain Reaction 755 .30 DNA Sequencing 785 .31 Analysis of Epigenetic Modifications 817 .32 Protein Nucleic Acid Interactions 831 .Part V Functional and Systems Analytics 873 .33 Sequence Data Analysis 875 .34 Analysis of Promoter Strength and Nascent RNA Synthesis 895 .35 Fluorescent In Situ Hybridization in Molecular Cytogenetics 917 .36 Physical and Genetic Mapping of Genomes 925 .37 DNA–Microarray Technology 945 .38 The Use of Oligonucleotides as Tools in Cell Biology 959 .39 Proteome Analysis 977 .40 Metabolomics and Peptidomics 1023 .41 Interactomics Systematic Protein Protein Interactions 1033 .42 Chemical Biology 1041 .43 Toponome Analysis 1057 .Appendix 1: Amino Acids and Posttranslational Modifications 1073 .Appendix 2: Symbols and Abbreviations 1075 .Appendix 3: Standard Amino Acids (three and one letter code) 1081 .Appendix 4: Nucleic Acid Bases 1083 .Index 1085

• ISBN: 978-3-527-33919-8
• Editorial: Wiley VCH
• Encuadernacion: Cartoné
• Páginas: 1134
• Fecha Publicación: 14/03/2018
• Nº Volúmenes: 1
• Idioma: Inglés