Planar Gel Electrophoresis: For Protein Separation presents the state-of-the-art in planar gel electrophoretic techniques and how they can be implemented in laboratory practice for protein separation. The book covers the whole of the subject, including its historical background, concepts, applications and new developments in planar gel electrophoresis in protein separation. Chapters are written by experts with deep experience in their field. This volume serves as a practical guide for researchers to choose and utilize suitable sample preparation procedures, also describing notable contemporary uses in planar gel electrophoresis in industrial and academic project environments. Contains the latest developments and applications of planar gel electrophoresis for protein separation Guides scientists in choosing the appropriate techniques to use to solve their problems, with an emphasis on the biological functions of the studies Provides an overview of each topic covered, with a focus on the big picture as well as the most relevant information for project planning INDICE: Section 1: History, Theory and Milestones1. Historical backgrounds and milestones2. Theory and core concepts3. Different gel chemistry and buffer systemsSection 2: Sample preparation4. Preparation of protein samplesSection 3: Different electrophoretic techniques5. SDS-PAGE (Denaturing gels)6. Non-denaturing gels7. Non-reducing gels8. Gradient gels to cover high MW and low MW proteins9. Separation of low MW peptides with Tris-Tricine gels10. Agarose Gels for Separating High MW Proteins11. Preparative gels12. Acid- urea - triton gel for separating baisc proteins and histones13. Blue native gels for separating protein complex14. Pore gradient gel electrophoresis (PGGE)15. Cationic Electrophoresis16. Isoelectric focusing (IEF)17. Immobilized pH gradients (IPG)18. Two-dimenstional gel electrophoresis (2-DE)19. Difference gel electrophoresis (DIGE)20. Diagonal Electrophoresis for disulfide bond detection21. Affinity gel electrophoresis (AEP)22. Electrophoretic mobility shift assays (EMSA)23. Immobilized metal affinity electrophoresis (IMAEP)24. 1D and 2D zymography (1DZ and 2DZ)25. SARCOSYL-PAGE: separation of PEGylated proteins26. Phenylboronate acrylamide gel electrophoresis for glycated proteins27. Field Inversion gel electrophoresis (FIGE)28. Microfluidic gel electrophoresisSection 4: Post-electrophoresis analysis29. Visualization of gel separated proteins with protein stains30. Densitometry analysis of protein bands or spots31. Data analysis software for extracting information from gel separations32. Recovery of proteins from gel with electroelution33. In-gel proteolytic digestion of proteins and mass spectrometrySection 5: Coulping electrophoresis with antibody detection34. Western Blotting35. Serological Proteome Analysis (SERPA)36. Immunofixation Electrophoresis (IFE)37. Immunoelectrophoresis (IEP)
- ISBN: 978-0-323-85473-3
- Editorial: Elsevier
- Encuadernacion: Rústica
- Páginas: 586
- Fecha Publicación: 01/11/2022
- Nº Volúmenes: 1
- Idioma: Inglés